Darrin Lemmer

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Multidrug-resistant Klebsiella pneumoniae producing the KPC carbapenemase have rapidly spread throughout the world, causing severe healthcare-associated infections with limited antimicrobial treatment options. Dissemination of KPC-producing K. pneumoniae is largely attributed to expansion of a single dominant strain, ST258. In this study, we explore(More)
BACKGROUND Minority resistant M. tuberculosis subpopulations can be associated with phenotypic resistance, but are poorly detected by Sanger sequencing or commercial molecular diagnostic assays. The role of targeted next generation sequencing (NGS) in resolving these minor variant subpopulations is unclear. METHODS We utilized Single Molecule-Overlapping(More)
The Staphylococcus aureus clonal lineage CC45 is a predominant colonizer of healthy individuals in northern Europe and constitutes a highly basal cluster of the S. aureus population. Here, we report the complete genome sequence of S. aureus strain CA-347 (NRS648), a representative of the methicillin-resistant USA600 clone predominantly found in the United(More)
Whole-genome sequencing (WGS) of bacterial isolates has become standard practice in many laboratories. Applications for WGS analysis include phylogeography and molecular epidemiology, using single nucleotide polymorphisms (SNPs) as the unit of evolution. NASP was developed as a reproducible method that scales well with the hundreds to thousands of WGS data(More)
Increasingly complex drug-resistant tuberculosis (DR-TB) is a major global health concern and one of the primary reasons why TB is now the leading infectious cause of death worldwide. Rapid characterization of a DR-TB patient's complete drug resistance profile would facilitate individualized treatment in place of empirical treatment, improve treatment(More)
1 Monitoring of Klebsiella pneumoniae. 2 3 Jolene R. Bowers,# Darrin Lemmer, Jason W. Sahl, Talima Pearson, Elizabeth M. 4 Driebe, David M. Engelthaler, Paul Keim. 5 6 Translational Genomics Research Institute, Flagstaff, AZ, USA; Northern Arizona 7 University, Flagstaff, AZ, USA 8 9 Running Head: Characterizing K. pneumoniae for Surveillance 10 11 #Address(More)
Health care-acquired infections (HAIs) kill tens of thousands of people each year and add significantly to health care costs. Multidrug-resistant and epidemic strains are a large proportion of HAI agents, and multidrug-resistant strains of Klebsiella pneumoniae, a leading HAI agent, have caused an urgent public health crisis. In the health care environment,(More)
Multidrug-resistant tuberculosis is a major public health dilemma. Heteroresistance, the co-existence of drug-resistant and drug-susceptible strains, or multiple drug-resistant strains with discrete haplotypes, may affect accurate diagnosis and institution of effective treatment. Subculture, or passage of cells onto fresh growth medium, is utilized to(More)
In Europe and the United States, the Staphylococcus aureus clonal lineage CC45 isolates are predominantly found in the nasal community of healthy individuals (1–7) and in large numbers in bloodstream infections (8, 9). There are two major geographically distinct sublineages of methicillin-resistant S. aureus (MRSA) CC45 found primarily in the United States(More)