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Cells continuously adapt to changing conditions through coordinated molecular and mechanical responses. This adaptation requires the transport of molecules and signaling through intracellular regions with differing material properties, such as variations in viscosity or elasticity. To determine the impact of regional variations on cell structure and(More)
Therapeutic ultrasound is widely employed in clinical applications but its mechanism of action remains unclear. Here we report prompt fluidization of a cell and dramatic acceleration of its remodeling dynamics when exposed to low intensity ultrasound. These physical changes are caused by very small strains (10(-5)) at ultrasonic frequencies (10(6) Hz), but(More)
Quantification of cellular responses to stimuli is challenging. Cells respond to changing external conditions through internal structural and compositional and functional modifications, thereby altering their transport and mechanical properties. By properly interpreting particle-tracking microrheology, we evaluate the response of live cells to cytoskeletal(More)
Direct-current, low-intensity, electric fields were suggested as a minimally invasive treatment for various cancers. The tumor microenvironment may affect treatment efficacy, albeit it has not generally been considered when evaluating novel anti-cancer treatments. We evaluate the effects of electric treatment on epithelial, breast-cancer cells, co-cultured(More)
We evaluated the transport of polymeric particles internalized into living cancer cells. The mean-square displacement demonstrates superdiffusion with a scaling exponent of 1.25. Scaling exponents of a range of displacement moments are bilinear with moment order, exhibiting slopes of 0.6 and 0.8. Thus, we present experimental evidence of strong anomalous(More)
During obesity development, preadipocytes proliferate and differentiate into new mature adipocytes, to increase the storage capacity of triglycerides. The morphology of the cells changes during differentiation from an elongated spindle-shape preadipocyte into a rounded, differentiated adipocyte. That change allows efficient packing of spheroidal(More)
Mechanics of cancer cells are directly linked to their metastatic potential, or ability to produce a secondary tumor at a distant site. Metastatic cells survive in the circulatory system in a non-adherent state, and can squeeze through barriers in the body. Such considerable structural changes in cells rely on rapid remodeling of internal structure and(More)
Cholesterol crystals are the building blocks of cholesterol gallstones. The exact structure of early-forming crystals is still controversial. We combined cryogenic-temperature transmission electron microscopy with cryogenic-temperature electron diffraction to sequentially study crystal development and structure in nucleating model and native gallbladder(More)
Obtaining reliable information on the physical state and ultrastructure of bile is difficult because of its mixed aqueous-lipid composition and thermodynamic metastability. We have used time-lapse cryogenic transmission electron microscopy (cryo-TEM) combined with video-enhanced light microscopy (VELM) to study microstructural evolution in nucleating bile.(More)