Objective-type total internal reflection fluorescence technique and Gaussian fit single particle tracking algorithm have been used to study the constrained Brownian motion characteristics of single fluorescent microspheres of 40 nm diameter in real-time. Three-dimensional tracking has been realized from the two-dimensional images by utilizing the… (More)
Using fast fluorescence micro-imaging technique, we have studied the real-time changes of intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) in S-nitrosoglutathione (GSNO)-induced mouse thymocyte early apoptosis. It was observed that [Ca<sup>2+</sup>]<sub>i</sub> increases rapidly until reaches a platform. The variety range and… (More)
Using a custom-made setup ICCD-DualView-TIRFM system, we have successfully performed two-dimensional imaging and tracking of fluorescence resonance energy transfer (FRET)-occurring single quantum dot and its energy acceptor Cy5. This work laid a foundation for the further application of quantum dot-FRET in life sciences.