Danying Lin

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Magnetic AC mode (MAC mode) atomic force microscopy (AFM), a novel type of tapping mode AFM in which the cantilever is driven directly by a magnetic field, is a powerful tool for imaging with high spatial resolution and better signal-to-noise in liquid environment. It may largely extend the application of AFM to living samples, especially those are(More)
Objective-type total internal reflection fluorescence technique and Gaussian fit single particle tracking algorithm have been used to study the constrained Brownian motion characteristics of single fluorescent microspheres of 40 nm diameter in real-time. Three-dimensional tracking has been realized from the two-dimensional images by utilizing the(More)
Cytoskeleton fibers form an intricate three-dimensional network to provide structure and function to microvessel endothelial cells. During accommodation to blood flowing, stress fiber bundles become more prominent and align with the direction of blood flow. This network either mechanically resists the applied shear stress (lateral force) or, if deformed, is(More)
Living organisms are generally composed of complex cellular processes which persist only within their native environments. To enhance our understanding of the biological processes lying within complex milieus, various techniques have been developed. Specifically, the emergence of super-resolution microscopy has generated a renaissance in cell biology by(More)
X-ray phase-contrast imaging based on grating interferometry is a technique with the potential to provide absorption, differential phase contrast, and dark-field signals simultaneously. The multi-line X-ray source used recently in grating interferometry has the advantage of high-energy X-rays for imaging of thick samples for most clinical and industrial(More)
Using a custom-made setup ICCD-DualView-TIRFM system, we have successfully performed two-dimensional imaging and tracking of fluorescence resonance energy transfer (FRET)-occurring single quantum dot and its energy acceptor Cy5. This work laid a foundation for the further application of quantum dot-FRET in life sciences.
A fluorescence microscopy imaging technique was applied to observe the single-cell kinetic changes of intracellular Ca2+ concentration ([Ca2+]i) and mitochondrial membrane potential (ΔΨm) during the early stage of S-nitrosoglutathione (GSNO)-induced thymocytes apoptosis. The kinetic features of [Ca2+]i and ΔΨm were quantitatively analyzed and compared by(More)
We propose a differential phase contrast imaging method in x-ray microscopy by utilizing a biased derivative filter, which is structurally similar to that used in visible optics, except that phase changes by the filter cannot be ignored in the x-ray range. However, it is demonstrated that the filter's phase retardation does not disturb its function of phase(More)
Using fast fluorescence micro-imaging technique, we have studied the real-time changes of intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) in S-nitrosoglutathione (GSNO)-induced mouse thymocyte early apoptosis. It was observed that [Ca<sup>2+</sup>]<sub>i</sub> increases rapidly until reaches a platform. The variety range and(More)
Grating-based X-ray differential phase contrast imaging (GDPCI) typically employs the phase-stepping technique to extract an object's phase information. This method requires heavy radiation dosage and is time consuming. Another potential approach is the reverse projection (RP) method, which, however, relies on a synchrotron radiation source to obtain highly(More)