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Hedgehog signalling in the mouse requires intraflagellar transport proteins
Genetic analysis shows that Wim, Polaris and the IFT motor protein Kif3a are required for Hedgehog signalling at a step downstream of Patched1 (the Hedgehog receptor) and upstream of direct targets of hedgehog signalling. Expand
Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds
Valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter and enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc. Expand
Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2
Valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4, and supports the possibility of reprograming through purely chemical means. Expand
Cilia and Hedgehog responsiveness in the mouse.
It is shown here that mouse embryos that lack both Ift172 and Smo are identical to IFT172 single mutants, which indicates that Ift 172 acts downstream of Smo, which means that IFT mutants have a weaker neural patterning phenotype than Smo mutants. Expand
A small-molecule inhibitor of tgf-Beta signaling replaces sox2 in reprogramming by inducing nanog.
High-content chemical screening is used to identify small molecules that can replace Sox2 in reprogramming and it is found that one of these molecules functions inReprogramming by inhibiting Tgf-beta signaling in a stable and trapped intermediate cell type that forms during the process. Expand
Signaling from Smo to Ci/Gli: conservation and divergence of Hedgehog pathways from Drosophila to vertebrates
Although the framework of the Hedgehog (Hh) signaling pathway is evolutionarily conserved, recent studies indicate that fundamental differences exist between Drosophila and vertebrates in the wayExpand
An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells.
The iCRISPR platform is uniquely suited for dissection of complex genetic interactions and pleiotropic gene functions in human disease studies and has the potential to support high-throughput genetic analysis in hPSCs. Expand
Mouse Dispatched homolog1 Is Required for Long-Range, but Not Juxtacrine, Hh Signaling
This work has identified recessive ENU-induced mutations in six genes that prevent normal specification of ventral cell types in the spinal cord, and positionally cloned the genes responsible for two of the mutant phenotypes, smoothened and dispatched, which are homologs of Drosophila Hh pathway components. Expand
All β Cells Contribute Equally to Islet Growth and Maintenance
The results support the conclusion that the β cell pool is homogeneous with respect to replicative capacity and suggest that all β cells are candidates for in vitro expansion. Expand
Genome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
Using TALEN and CRISPR/Cas-mediated gene editing and hPSC-directed differentiation for a systematic analysis of the roles of eight pancreatic transcription factors revealed a number of previously unsuspected developmental mechanisms with implications for type 2 diabetes. Expand