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Microscope imaging technologies have turned out to yield an indispensable tool in modern biomedical research. Combined with fluorescence labeling techniques they not only provide new perspectives on tissues and cells as a whole, but also on processes at the cellular level, and will be one important experimental technique of systems biology. To handle this(More)
MicroRNAs (miRNAs) control gene expression at the post-transcriptional level. However, the identification of miRNAs regulating the fate of a specific messenger RNA remains limited due to the imperfect complementarity of miRNAs and targeted transcripts. Here, we describe miTRAP (miRNA trapping by RNA in vitro affinity purification), an advanced protocol of(More)
The function of complex networks in the nervous system relies on the proper formation of neuronal contacts and their remodeling. To decipher the molecular mechanisms underlying these processes, it is essential to establish unbiased automated tools allowing the correlation of neurite morphology and the subcellular distribution of molecules by quantitative(More)
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