Danielle Lawrence

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Thermosensation is an essential sensory function that is subserved by a variety of transducer molecules, including those from the Transient Receptor Potential (TRP) ion channel superfamily. One of its members, TRPM8 (CMR1), a ligand-gated, nonselective cation channel, is activated by both cold and chemical stimuli in vitro. However, its roles in cold(More)
Experiments were done in urethane anesthetized rats to identify single units in the region of the parabrachial nucleus (PBN) projecting directly to 'cardiovascular' responsive sites in either the paraventricular nucleus of the hypothalamus (PVH) or the supraoptic commissure and nucleus (SOC-SON) region. Fifty-five single units were antidromically activated(More)
In lactating, urethane-anaesthetized female rats extracellular recordings were made from paraventricular nucleus (PVN) neurones that were antidromically activated following electrical stimulation of the neurohypophysis, amygdala or nucleus tractus solitarius/vagal complex (NTS/VC). Of the PVN units, 98 projected to the neurohypophysis but none of these(More)
The hypothalamic paraventricular nucleus is known to send projections to the dorsal medullary area where it appears to innervate the nucleus tractus solitarius and the dorsal motor nucleus of the vagus. Experiments were carried out in urethane anesthetized rats to identify single vagal motor neurons identified by antidromic invasion and to determine their(More)
The present study describes the characterization of an opioid binding site on membranes prepared from the R1.1 cell line, a murine thymoma. Specific (-)[3H]bremazocine binding was saturable, stereoselective, and limited to a single high affinity binding site with a Kd value of 15.2 +/- 1.6 pM and a Bmax value of 54.8 +/- 6.0 fmol/mg of protein. The(More)
Arginine-vasopressin (AVP) has been implicated as a putative central neurotransmitter or neuromodulator in some brain functions. This study demonstrates binding of [3H]AVP to rat brain homogenates that is pH and temperature dependent, is saturable (Kd = 0.77 nM, Bmax = 0.374 pmol/mg) and reversible. A number of AVP analogues competitively displaced the(More)
Vasopressin causes motor disturbances following intracerebroventricular injections and a second central injection results in an apparent sensitization of the animal to the convulsant effect of vasopressin. In this study, the effect of vasopressin pretreatment on the central and peripheral pressor effects of vasopressin was examined in Sprague-Dawley rats.(More)
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