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Genetic analysis of xenocoumacin antibiotic production in the mutualistic bacterium Xenorhabdus nematophila
Findings suggest that OmpR‐phosphate negatively regulates xcnA–L gene expression while positively regulating xcnMN expression, and Interestingly, inactivation of ompR enhanced cell viability during prolonged culturing. Expand
Determination of the absolute configuration of peptide natural products by using stable isotope labeling and mass spectrometry.
A combination of stable isotope labeling of Photorhabdus and XenorhabDus strains and their transaminase mutants followed by detailed MS analysis enabled the structure elucidation of novel cyclopeptides named GameXPeptides including their absolute configuration in crude extracts without their actual isolation. Expand
A New Type of Pyrrolidine Biosynthesis Is Involved in the Late Steps of Xenocoumacin Production in Xenorhabdus nematophila
This work could identify xenocoumacins (XCNs) I and II (1 and 2, respectively, in Scheme 1), which were isolated several years ago from different strains of Xenorhabdus nematophila, which are thought to be involved in killing bacteria living inside the insect gut, where these bacteria would compete with XenoACHTUNGTRENNUNGrhabDus for food in the dead insect. Expand
Xenortide Biosynthesis by Entomopathogenic Xenorhabdus nematophila.
The structure of xenortide D was elucidated using a combination of labeling experiments followed by MS analysis and was confirmed by synthesis, and a weak activity of tryptamine-carrying xenortides against Plasmodium falciparum and Trypanosoma brucei was revealed. Expand
A natural prodrug activation mechanism in nonribosomal peptide synthesis.
We have identified a new mechanism for the cleavage and activation of nonribosomally made peptides and peptide-polyketide hybrids that are apparently operational in several different bacteria. ThisExpand
Rhabdopeptides as Insect‐Specific Virulence Factors from Entomopathogenic Bacteria
Six novel linear peptides, named “rhabdopeptides”, have been identified in the entomopathogenic bacterium Xenorhabdus nematophila and detailed analysis of an rdp mutant revealed that these compounds participate in virulence towards insects and are produced upon bacterial infection of a suitable insect host. Expand
A natural prodrug activation mechanism in the biosynthesis of nonribosomal peptides.
Highlight describes the recently discovered prodrug activation mechanism found in the biosynthesis of nonribosomally produced peptides and peptide/polyketide hybrids as well as related mechanisms.
Biosynthesis of the Antibiotic Nematophin and Its Elongated Derivatives in Entomopathogenic Bacteria.
Six extended nematophin derivatives from Xenorhabdus PB62.4 carrying an additional valine are reported, and preliminary bioactivity studies suggest a biological role of these compounds in the bacteria-nematode-insect symbiosis. Expand
Thiol Probes To Detect Electrophilic Natural Products Based on Their Mechanism of Action.
A reactivity-guided drug discovery approach for electrophilic natural products, a therapeutically relevant class of natural products that covalently modify their cellular targets, in crude extracts using carefully designed halogenated aromatic reagents. Expand