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Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium
TLDR
Although both Tribolium and C. elegans show a robust systemic RNAi response, the genome-wide survey reveals significant differences between the RNAi mechanisms of these organisms. Expand
The genome of the model beetle and pest Tribolium castaneum
Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model organism for the study of generalized insect development, and an important pest of stored agriculturalExpand
RNAi in the red flour beetle (Tribolium).
TLDR
This protocol describes the production of dsRNA from a polymerase chain reaction (PCR) template, injection procedures for each Tribolium life stage, and important controls for effective analysis. Expand
iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum
TLDR
The iBeetle-Base makes available annotations of RNAi phenotypes, which were gathered in a large scale RNAi screen in the red flour beetle Tribolium castaneum (i beetle screen), and provides access to sequence information and links for all Tribolial genes. Expand
Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target
TLDR
It is shown that RNAi target genes do not appear to act synergistically and that protein sequence conservation does not correlate with the number of potential off target sites, and the identified GO term combinations will help to identify efficient target genes from organ specific transcriptomes. Expand
Wnt/β-catenin signaling integrates patterning and metabolism of the insect growth zone
TLDR
The short germ beetle Tribolium castaneum is used to investigate two Wnt and Hh signaling centers located in the head anlagen and in the growth zone of early embryos and it is found that Wnt/β-catenin signaling acts upstream of Hh in thegrowth zone, whereas the opposite interaction occurs in theHead. Expand
Egfr signaling regulates distal as well as medial fate in the embryonic leg of Tribolium castaneum.
TLDR
It is shown that Egfr signaling has a more complex role in T. castaneum than in D. melanogaster and is not only required in the distal leg, but is also involved in formation of the medial leg (including femur and tibia), providing a possible explanation why this role is less evolutionarily conserved in the insects. Expand
The Tribolium ortholog of knirps and knirps-related is crucial for head segmentation but plays a minor role during abdominal patterning.
TLDR
The findings support the notion that the role of gap gene homologs in abdominal segmentation differs fundamentally in long and short germ insects and suggests an ancestral role for knirps as head patterning gene. Expand
Separable functions of wingless in distal and ventral patterning of the Tribolium leg
TLDR
The role of wg in leg development of the flour beetle Tribolium castaneum is studied and stage-specific staggered embryonic RNAi in wild-type and transgenic EGFP expressing enhancer trap lines are used to demonstrate separable functions. Expand
The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology
TLDR
The iBeetle screen is presented, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology and transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila. Expand
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