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Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium
- Y. Tomoyasu, Sherry Miller, S. Tomita, M. Schoppmeier, Daniela Grossmann, Gregor Bucher
- BiologyGenome Biology
- 17 January 2008
Although both Tribolium and C. elegans show a robust systemic RNAi response, the genome-wide survey reveals significant differences between the RNAi mechanisms of these organisms.
The genome of the model beetle and pest Tribolium castaneum
Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model organism for the study of generalized insect development, and an important pest of stored agricultural…
RNAi in the red flour beetle (Tribolium).
- Nico Posnien, Johannes B. Schinko, Daniela Grossmann, T. Shippy, Barbora Konopová, Gregor Bucher
- BiologyCold Spring Harbor protocols
- 1 August 2009
This protocol describes the production of dsRNA from a polymerase chain reaction (PCR) template, injection procedures for each Tribolium life stage, and important controls for effective analysis.
iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum
The iBeetle-Base makes available annotations of RNAi phenotypes, which were gathered in a large scale RNAi screen in the red flour beetle Tribolium castaneum (i beetle screen), and provides access to sequence information and links for all Tribolial genes.
Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target
It is shown that RNAi target genes do not appear to act synergistically and that protein sequence conservation does not correlate with the number of potential off target sites, and the identified GO term combinations will help to identify efficient target genes from organ specific transcriptomes.
The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology
The iBeetle screen is presented, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology and transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila.
The Tribolium ortholog of knirps and knirps-related is crucial for head segmentation but plays a minor role during abdominal patterning.
Separable functions of wingless in distal and ventral patterning of the Tribolium leg
- Daniela Grossmann, Johannes Scholten, Nikola-Michael Prpic
- BiologyDevelopment Genes and Evolution
- 19 December 2009
The role of wg in leg development of the flour beetle Tribolium castaneum is studied and stage-specific staggered embryonic RNAi in wild-type and transgenic EGFP expressing enhancer trap lines are used to demonstrate separable functions.
Wnt/β-catenin signaling integrates patterning and metabolism of the insect growth zone
- Georg Oberhofer, Daniela Grossmann, J. Siemanowski, T. Beißbarth, Gregor Bucher
- 15 December 2014
The short germ beetle Tribolium castaneum is used to investigate two Wnt and Hh signaling centers located in the head anlagen and in the growth zone of early embryos and it is found that Wnt/β-catenin signaling acts upstream of Hh in thegrowth zone, whereas the opposite interaction occurs in theHead.