Daniel Summerer

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KEYWORDS: , ,'DNA damage • DNA polymerases • DNA replication • thymidine dimers • UV light Ultraviolet (UV) light causes a variety of damage in DNA. The most abundant lesions are pyrimidine dimers such as the pyrimidine (6-4) pyrimidone photo-product (6-4PP) and the eis,syn cyclo-butane-pyrimidine dimer ((PD)11J (Scheme 1). These lesions are often repaired(More)
The fidelity of DNA polymerase activity is of central importance for numerous biotechnological applications.r1.2 1 The imperfect fidelity of DNA polymerases under the unnatural conditions of several techniques such as those of the polymerase chain reaction (PCR) either restricts the application of these enzymes or demands their tedious optimization. Thus, a(More)
We genetically encoded the photocaged amino acid 4,5-dimethoxy-2-nitrobenzylserine (DMNB-Ser) in Saccharomyces cerevisiae in response to the amber nonsense codon TAG. This amino acid was converted to serine in living cells by irradiation with relatively low-energy blue light and was used to noninvasively photoactivate phosphorylation of the transcription(More)
Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of(More)
Faithful transfer of genetic information from one generation to its offspring is crucial for the survival of any living species, Genomic integrity relies greatly upon the ability of DNA polymerases to efficiently catalyze selective DNA synthesis in a template-directed manner,11J Despite enormous efforts in structural and functional studies, the complex(More)
DNA polymerases are the key enzymes for DNA synthesis involved in DNA replication, recombination, and repair. These enzymes undergo manifold contacts with the primer-template-substrates reaching up to several nucleotide pairs beyond the catalytic centre. To evaluate these enzyme contacts with the DNA substrates we applied novel synthetic steric probes in(More)
The unnatural amino acid p-nitrophenylalanine (pNO2-Phe) was genetically introduced into proteins in Escherichia coli in response to the amber nonsense codon with high fidelity and efficiency by means of an evolved tRNA/aminoacyl-tRNA synthetase pair from Methanocuccus jannaschii. It was shown that pNO2-Phe efficiently quenches the intrinsic fluorescence of(More)
It has been known for almost twenty years that nucleic acids may promote chemical reactions. Catalytic nucleic acids can be found in nature or evolved through in vitro selection processes.[ll Structural and functional investigations suggest that catalytically active nucleic acids act in a similar manner to enzymes: they promote chemical reactions through(More)
Recently, 4'C-ethynyl nucleoside analogues have been identified as highly potent agents against HIV-1, including several multidrug-resistant strains. In contrast to most known nucleoside inhibitors 4'C-ethynyl nucleoside analogues possess a 3'-hydroxyl function. Here we show that the 5'O-triphosphate of 4'C-ethynyl thymidine gets readily incorporated into a(More)