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When induced by in vivo oestrogen stimulation, ciliogenesis continues in culture in vitro of quail oviduct implants. Ultrastructure of ciliogenic cells was compared after culture for 24 or 48 h in the presence or absence of 10(-5) M-taxol. Taxol, which promotes polymerization and stabilization of microtubules, disturbed ciliogenesis, but formation of basal(More)
The diversity of microtubular networks was analyzed in quail oviduct and in Paramecium cells using conventional and confocal immunofluorescence as well as pre- and post-embedding EM immunocytochemistry with a variety of anti-tubulin antibodies. The 6-11B-1 monoclonal antibody, specific for the post-translational acetylation of Lys 40 of alpha-tubulin, and a(More)
Basal bodies from laying quail oviduct were semipurified and used as immunogen to produce monoclonal antibodies. On 38 clones obtained and among those staining the apical pole of the ciliated cell, CC-310 was chosen because it labeled the apical region with a punctuated aspect, suggesting a staining of basal bodies or of basal body-associated structures;(More)
The hormonal control of ciliogenesis and transformation of mucous cells was studied in the oviduct (magnum) of ovariectomized quails. Estradiol benzoate induces ciliogenesis with doses varying from 10 mug/day to 100 mug/day after 6 days of treatment. With 100 mug/day, differentiation of some mucous cells is also induced as well as the formation of(More)
Localization of mineral elements in the shell of quail eggs was studied by secondary ion mass spectrometry (SIMS) and electron probe microanalysis (EPMA). Normal eggs as well as eggs from hens intoxicated with strontium were studied. A simple preparative technique was employed that is advantageous for the study of mineralized tissues. Semithin (1 µm)(More)
Oviduct implants from quails which were primarily stimulated in vivo by estrogen so as to induce ciliogenesis in some epithelial cells were cultured in vitro in the presence or absence of colchicine or nocodazole. After 24 or 48 hr of culture, implants were examined by transmission and scanning electron microscopy to determine drug-induced alterations in(More)
The different steps of ciliogenesis occurring in quail oviduct were compared to the ciliogenesis pattern described in other metazoan species. Centrioles are generated according to pathways that are found within the same cell: the centriolar and the acentriolar pathways. In the acentriolar pathway, centrioles are generated in the Golgi area, without contact(More)
The ultrastructures of mucous cell from mouse colon and quail oviduct were revisited after quick-freezing followed by cryosubstitution and compared with results from standard fixation. After quick-freezing, the mucous cells of both models do not show the classical aspect of the goblet cell. The secretory granules which contain a dense product do not swell(More)
Ciliated cells are characterized by a highly organized cytoskeleton which is connected with the ciliary apparatus. The organization of microtubules, microfilaments, and cytokeratin filaments is described and the relationships of each network with the ciliary apparatus are emphasized. Possible functions of such a complex cytoskeleton are discussed.