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Mechanistic analysis of ghrelin-O-acyltransferase using substrate analogs.
TLDR
Ghrelin substrates with amino-alanine in place of Ser3 demonstrated that GOAT can catalyze the formation of an octanoyl-amide bond at a similar rate compared with the natural reaction, and the conserved His338 residue was required both for Ser3 and amino-Ala3 ghrelin substrateates, suggesting that His338 may have a key catalytic role beyond that of a general base. Expand
Drosophila melanogaster as a model system to study long‐chain fatty acid amide metabolism
TLDR
It is demonstrated that Drosophila melanogaster is an excellent model system for the study long‐chain fatty acid amide metabolism as it is quantified the endogenous levels of N‐acylglycines, N‐ACYldopamines, N-acylethanolamines, and primary fatty Acid amides by LC/QTOF‐MS. Expand
Akt Kinase Activation Mechanisms Revealed Using Protein Semisynthesis
TLDR
It is determined that pSer473-Akt activation is driven by an intramolecular interaction between the C-tail and the pleckstrin homology (PH)-kinase domain linker that relieves PH domain-mediated Akt1 autoinhibition. Expand
A Tunable Brake for HECT Ubiquitin Ligases.
TLDR
The results clarify the mechanisms of several HECT protein cancer associated mutations and provide a new framework for understanding how HECT ubiquitin ligases must be finely tuned to ensure normal cellular behavior. Expand
Identification of an arylalkylamine N‐acyltransferase from Drosophila melanogaster that catalyzes the formation of long‐chain N‐acylserotonins
TLDR
Identification of endogenous amounts of N‐acylserotonins and colocalization of these fatty acid amides and AANATL2 transcripts gives supporting evidence that AANatL2 has a role in the biosynthetic formation of these important cell signalling lipids. Expand
Mechanistic and Structural Analysis of Drosophila melanogaster Arylalkylamine N-Acetyltransferases
TLDR
A combination of pH–activity profiling and site-directed mutagenesis to study prospective residues believed to function in AANATA catalysis led to an assignment of Glu-47 as the general base in catalysis with an apparent pKa of 7.0. Expand
Glycine N-acyltransferase-like 3 is responsible for long-chain N-acylglycine formation in N18TG2 cells[S]
TLDR
Heavy-labeled precursor and subtraction lipidomic experiments in mouse neuroblastoma N18TG2 cells, a model cell line for the study of fatty acid amide metabolism, establish the biosynthetic pathways for the N-acylglycines and the PFAMs. Expand
N-acylethanolamines as novel alcohol dehydrogenase 3 substrates.
TLDR
NAEs were oxidized by ADH3, yielding the N-acylglycinals as the product, and data show that the NAEs can serve as alcohol dehydrogenase substrates. Expand
Structural and Mechanistic Analysis of Drosophila melanogaster Agmatine N-Acetyltransferase, an Enzyme that Catalyzes the Formation of N-Acetylagmatine
TLDR
Evidence is provided that Drosophila melanogaster AgmNAT catalyzes the formation of N-acetylagmatine using an ordered sequential mechanism; acetyl-CoA binds prior to agmatine to generate an Agm NAT•acetyl- CoA•agmatines ternary complex prior to catalysis. Expand
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