Daniel H. Butler

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Our previous amino acid substitutions at the postulated lipid-exposed transmembrane segment M4 of the Torpedo californica acetylcholine receptor (AChR) focused on the alpha C418 position. A tryptophan substitution on the alpha C418 produced a 3-fold increase in normalized macroscopic response to acetylcholine in voltage-clamped Xenopus laevis oocytes (Lee(More)
Using Fourier-transform infrared resonance spectroscopy, we examined the structure of the purified Torpedo californica nicotinic acetylcholine receptor in reconstituted dioleoylphosphatidylcholine membranes in H2O and D2O. Using the amide-I band, we calculated the secondary structure of nAChR in H2O to be approx. 19% alpha-helix, 42% beta-structure, 24%(More)
Previous studies examining the relationship between cognition and ability to benefit from inpatient rehabilitation have found cognitive dysfunction to be associated with a poor rehabilitation outcome. To examine whether cognitive dysfunction precluded effective rehabilitation, 52 consecutive admissions to a geriatric rehabilitation unit were assigned Mini(More)
Permselective acrylic membranes were employed to prevent immune rejection of discordant islet xenografts isolated from various large animals. Canine, porcine, and bovine islets were seeded into tubular diffusion chambers and transplanted into the peritoneum of 27 nonimmunosuppressed streptozotocin-induced diabetic Lewis rats. Six recipients received islet(More)
1. To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function, six mouse–Torpedo chimeric α-subunits were constructed (Fig. 2) and coexpressed with Torpedo californica β-, γ-, and δ-subunits in Xenopus laevis oocytes. 2. nAChRs containing a chimeric α-subunit were examined by voltage- and patch-clamp(More)
Patch-clamping and photoaffinity-labeling techniques were used to study the effects of binding of monoclonal antibodies (mAbs) on the function of Torpedo californica nicotinic acetylcholine receptor (nAChR). The rat anti-Torpedo nAChR mAbs examined here are known to inhibit ligand binding to either the high-affinity (mAb 247) or both the high- and(More)
Tarsal tunnel syndrome is a commonly considered compression of the tibial nerve and its plantar divisions as the nerve curves behind the medial malleolus underneath the flexor retinaculum. Motor, sensory, and/or mixed-nerve conduction studies are used to confirm or exclude the presence of compression of the posterior tibial nerve and its plantar divisions.(More)
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