Daniel Głód

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The composition of C-glycosylflavones in fenugreek seeds is complicated and comprises of flavone mono- and di-C-glycosides, derivatives of both apigenin and luteolin as well as their ester forms. In this paper the application of one- and two-dimensional HPLC techniques: off-line heart-cutting and on-line comprehensive for the analysis of flavone(More)
Raspberries, derived from different cultivar varieties, are a popular ingredient of everyday diet, and their biological activity is a point of interest for researchers. The ethanol-water extracts from four varieties of red (Rubus idaeus'Ljulin', 'Veten', 'Poranna Rosa') and black (Rubus occidentalis'Litacz') raspberries were evaluated in the range of their(More)
INTRODUCTION Salicis Cortex, made from willow bark is a herbal remedy, which is standardised based on the content of salicin, a compound with analgesic and antiphlogistic properties. However, clinical trials suggest that other compounds also present in Salicis Cortex can contribute to the pharmacological effects. OBJECTIVE To characterise the composition(More)
Hairy root cultures of Scutellaria lateriflora were established using Agrobacterium rhizogenes A4 and produced acteoside (18.5 mg g−1 dry wt), baicalin (14.5 mg g−1 dry wt) and wogonoside (12 mg g−1 dry wt). Yeast extract (50 μg ml−1) increased acteoside production 1.4-fold and flavone production 1.7-fold after 7 and 14 days of elicitation. Addition of(More)
Staphylococcus aureus is the most common infectious agent involved in the development of skin infections that are associated with antibiotic resistance, such as burn wounds. As drug resistance is a growing problem it is essential to establish novel antimicrobials. Currently, antibiotic resistance in bacteria is successfully controlled by multi-drug(More)
The separation of anthocyanins present in the fruits of 11 varieties of red raspberries (Rubus idaeus L.) was performed by high performance liquid chromatography (HPLC) with a diode-array detector and evaporative light scattering detection (ELSD). The ELSD parameters--drift tube temperature, nebulising gas flow rate and gain value--were optimised to get the(More)
The SPE-HPLC method was developed to determine an isosalipurposide (5) and its derivative, 6"-O-p-coumaroyl ester (6) in the bark of eight taxa (I-VIII) belonging to three species of the genus Salix and originating from a natural habitat or cultivated for pharmaceutical purposes. The chalcones were separated by HPLC under gradient elution with the(More)
This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ,(More)
BACKGROUND The young shoots of Rubus idaeus are traditionally used as a herbal remedy in common cold, fever and flu-like infections yet there is no research concerning this plant material. The aim of the study was to evaluate the chemical composition and biological properties of raspberry shoots from 11 cultivar varieties. METHODS The methanol extracts(More)
Lonicera caerulea is a shrub native to the Northen Hemisphere, with its fruits having a long tradition of being used in traditional medicine. The flowers, although a potential source of diverse phenolic compounds have not been studied in terms of phenolic content. In this paper, a 2D LC heart-cutting system, operating in both on-line and off-line modes, was(More)