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Mg(2+) plays important roles in numerous cellular functions. Mitochondria take part in intracellular Mg(2+) regulation and the Mg(2+) concentration in mitochondria affects the synthesis of ATP. However, there are few methods to observe Mg(2+) in mitochondria in intact cells. Here, we have developed a novel Mg(2+)-selective fluorescent probe, KMG-301, that… (More)
This review summarizes progress in the field of near-infrared (NIR) fluorophores achieved during the last decade from the viewpoint of chemistry. Those compounds are of wide interest in bioanalysis and bioimaging, such as in vivo fluorescence imaging. Particular focus is placed on the recent developments of BODIPY and rhodamine derivatives, which belong to… (More)
Three novel coelenterazine (CTZ) derivatives with extension at the C-6 position of the imidazopyrazinone structure show significant bioluminescence emission with known renilla luciferase variants, indicating a promising method to develop CTZ derivatives with superior optical properties compared to hitherto reported compounds.
This communication reports the synthesis, chemiluminescence properties, and biological application of KEIO-BODIPY-imidazopyrazine (KBI), a yellow-green chemiluminescent probe for the detection of reactive oxygen species (ROS) generated from living cells.
This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking… (More)
A novel, smart, chemical taste sensor that realistically mimics the behavior of the human gustatory system is described. The taste sensor consists of an array of electrochemical sensors that represent the gustatory receptors on the human tongue, and a two-phase optimized radial basis function network (RBFN) to represent the human brain, which… (More)
We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a… (More)