Daniel C. Uchôa

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Glycerol is the cryoprotector most frequently used to freeze semen from different species. The objective of the present study was to compare the effect of single and fractionated glycerol addition on canine semen quality after thawing. Sperm fractions from 12 stud dogs were collected, evaluated, extended in Tris plus egg-yolk and separated into two aliquots(More)
The aim of this study was to evaluate powdered coconut water extender (ACP-106c; ACP Serviços Tecnológicos Ltda, ACP Biotecnologia, Fortaleza, Ceará, Brazil) as a diluent for freezing dog semen and the fertility after vaginal insemination of semen frozen therein. Ten ejaculates were collected from five dogs, evaluated fresh, diluted in ACP-106c, 10% egg(More)
The objective of this study was to describe the sexual characteristics of domestic queens kept under natural equatorial photoperiod conditions without mating. Estrous signs were detected in 25 pubertal queens by manual stimulation and by exposure to a tomcat twice daily for 6 months (January to June). The signs observed were tail deflection, spinal flexion,(More)
Semen was collected by digital manipulation from six adult dogs. The second fraction of the ejaculate was used in this study. The semen was assessed by macroscopic and microscopic criteria 1 min after collection, first dilution, cooling, glycerol addition and thawing. Experiments were conducted to compare the effect of three different concentrations of(More)
Overlay networks are considered an easy and effective way to deploy value-added Internet services by extending the best-effort delivery service supported by IP layer. However, a careful design is needed in order to take advantage of these benefits. Since there are no standards for overlay networks yet, they are often independently designed, which means that(More)
Background: Canine leishmaniasis (CanL) is caused by an obligatory intracellular parasite of Leishmania genus that affects organs and tissues. Several studies evaluate the role of reactive oxygen species (ROS) in the pathogenesis of many diseases. The overproduction of ROS on infectious diseases can induce an imbalance between oxidants and antioxidants at(More)
The objective was to assess the effect of adding various concentrations of dimethylformamide on characteristics of canine semen diluted in powdered coconut water (ACP-106C; ACP Biotecnologia, Fortaleza, CE, Brazil) and frozen at -196°C. Fifteen ejaculates were collected by manual stimulation from five adult Boxer dogs. The sperm-rich fraction was diluted in(More)
The objective was to determine the effect of powdered coconut water extender (ACP-106c) on the proportion of female puppies born. Twenty French Bulldog bitches were subjected to natural mating (NM) and, during the subsequent two estrus periods, were bred by intravaginal artificial insemination (AI), using chilled semen (from the same males) diluted in(More)