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Causes and Effects of N-Terminal Codon Bias in Bacterial Genes
Exploiting Redundancy The genetic code is redundant—multiple codons can code for the same amino acid. So-called synonymous codon changes within genes can nonetheless have substantial affects onExpand
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Genomically Recoded Organisms Expand Biological Functions
Changing the Code Easily and efficiently expanding the genetic code could provide tools to genome engineers with broad applications in medicine, energy, agriculture, and environmental safety. LajoieExpand
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Composability of regulatory sequences controlling transcription and translation in Escherichia coli
The inability to predict heterologous gene expression levels precisely hinders our ability to engineer biological systems. Using well-characterized regulatory elements offers a potential solutionExpand
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Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement
Template-mediated, genome construction and assembly created a strain with 80 precise codon changes. We present genome engineering technologies that are capable of fundamentally reengineering genomesExpand
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Design, synthesis, and testing toward a 57-codon genome
Recoding and repurposing genetic codons By recoding bacterial genomes, it is possible to create organisms that can potentially synthesize products not commonly found in nature. By systematicExpand
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A Multiplexed Assay for Exon Recognition Reveals that an Unappreciated Fraction of Rare Genetic Variants Cause Large-Effect Splicing Disruptions.
Mutations that lead to splicing defects can have severe consequences on gene function and cause disease. Here, we explore how human genetic variation affects exon recognition by developing aExpand
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Rational optimization of tolC as a powerful dual selectable marker for genome engineering
Selection has been invaluable for genetic manipulation, although counter-selection has historically exhibited limited robustness and convenience. TolC, an outer membrane pore involved inExpand
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Emergent rules for codon choice elucidated by editing rare arginine codons in Escherichia coli
Significance This work presents the genome-wide replacement of all rare AGR (AGA and AGG) arginine codons in the essential genes of Escherichia coli with synonymous CGN alternatives. Synonymous codonExpand
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DNAplotlib: Programmable Visualization of Genetic Designs and Associated Data.
DNAplotlib ( www.dnaplotlib.org ) is a computational toolkit for the programmable visualization of highly customizable, standards-compliant genetic designs. Functions are provided to aid with bothExpand
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Product length, dye choice, and detection chemistry in the bead-emulsion amplification of millions of single DNA molecules in parallel.
The amplification of millions of single molecules in parallel can be performed on microscopic magnetic beads that are contained in aqueous compartments of an oil-buffer emulsion. These bead-emulsionExpand
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