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Hsp70 can stimulate cells of the innate immune system directly by acting as "danger"-signaling molecules. To understand the immune defense mechanisms of swimming crab Portunus trituberculatus (Decapoda: Brachyura: Portunidae), the cDNA of Hsp70 (designated PtHsp70) was cloned by the combination of homology cloning and rapid amplification of cDNA ends(More)
Serine proteinase homologues (SPHs), as one of prophenoloxiase-activating factors (PPAFs), play critical roles in innate immunity of crabs. Based on an EST from the eyestalk full length cDNA library, the complete cDNA (designated as PtSPH) and genomic DNA of SPH from the swimming crab Portunus trituberculatus were cloned in this study. The estimated(More)
Microsatellites have been a powerful genetic tool in determining pedigree relationships among individuals. A microsatellite marker suite can be used to reliably assign parentage in the Portunus trituberculatus selective breeding programs. Thirty published microsatellites were screened for their suitability to be included in a DNA parentage marker suite.(More)
This article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber(More)
Thirty polymorphic microsatellite markers for swimming crab P. trituberculatus are developed from EST sequences, genomic libraries and cross-species amplification in P. pelagicus, respectively. They are characterized using 24 P. trituberculatus individuals from Yingkou natural population. The number of alleles per locus range from 4 to 21 with a mean number(More)
Reliable and real-time information on soil and crop properties is important for the development of management practices in accordance with the requirements of a specific soil and crop within individual field units. This is particularly the case in salt-affected agricultural landscape where managing the spatial variability of soil salinity is essential to(More)
We describe a compact double-layer waveguide grating splitter that not only achieves efficient coupling between single mode fiber and a silicon-on-insulator optical waveguide but also realizes effective splitting. By appropriate choice of waveguide/grating parameters, including thicknesses, periods, height, and fill factor to optimize the mode matching,(More)
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