Dana Mackenzie

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The sequence of 3553 nucleotides corresponding to the 3'-terminal region of potato virus S (PVS) has been determined from cloned cDNA. The sequence obtained contains six open reading frames (ORFs) encoding proteins of Mr 10,734, Mr 32,515, Mr 7,222, Mr 11,802, Mr 25,092 and at least Mr 41,052. The sequence of the 33K ORF has been confirmed to be that of the(More)
A recombinant plasmid containing the entire tomato spotted with virus (TSWV) nucleocapsid gene, with the exception of nucleotide encoding three N-terminal amino acids, was isolated by screening a complementary DNA library, prepared against random primed viral RNA, using a specific monoclonal antibody. The insert contained in plasmid pTSW1 was repaired and(More)
The reactivity of six monoclonal antibodies with native southern bean mosaic virus particles, swollen virus, swollen virus contracted by divalent cations or by pH adjustment to 5.0, and virus coat protein at pH 7.5 and 5.0 were assessed by antigen inhibition enzyme-linked immunosorbent assays. Clones B4, B7, and B11 did not react with native virus but were(More)
The coat protein gene from potato virus S (PVS) was introduced into Nicotiana debneyii by leaf disc transformation using Agrobacterium tumefaciens. Transgenic plants expressing the viral coat protein were highly resistant to subsequent infection by the ME strain of PVS as indicated by an absence of symptom development and a lack of accumulation of virus in(More)
A first attempt to gather the available information on subacute sclerosing panencephalitis (SSPE) in Southern Africa has produced records of 79 cases which have occurred since 1955. An important feature of the epidemiology is the clustering of 48 cases in the 5-year period 1970-1974. Thirty of these patients were resident in the Cape Province, and most of(More)
An analysis of cases of subacute sclerosing panencephalitis (SSPE) in Southern Africa to the end of 1976 is presented. In the 2 years since the last report in 1975, 15 new cases have been added to the register. Seven patients from the Cape Province were investigated in Groote Schuur and Red Cross War Memorial Children's Hospitals in Cape Town and a recent(More)
Moving beyond the qualitative conclusions of earlier models in biology, mathematical models are beginning to make quantitative, testable predictions about real patients. When Renee Fister was three years old, she lost her younger brother, then 18 months old, to cancer. Growing up, she hoped to go to medical school so that she could fight the disease that(More)
Subacute sclerosing panencephalitis can no longer be regarded as a rare disease in all parts of southern Africa. Information derived from a third postal survey added to that from our own experience in Cape Town has brought to light 116 cases; 61 of the patients came from the Cape Province, 40 from the Transvaal and the remaining 15 from the other regions.(More)