Dan-ying Tao

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OBJECTIVES To analyze the status quo of dental erosion in 3- to 6-year-old children in Shanghai. METHODS A stratified, cluster, multistage random sampling methods was applied to 3- to 6-year-old children in Shanghai in 2012. Both questionnaire and clinical oral examination were performed in the survey. The questionnaire included general information, such(More)
To help dental students acquaint the medical environment, doctor-patient communication and relationship, early clinic education was arranged in our college of stomatology. The interesting topics were chosen to enhance the learning enthusiasm of the students in the teaching practice of preventive dentistry. Students were encouraged to practice the skill of(More)
PURPOSE To obtain the clone of Streptococcus salivarius 57.I urease genes ureIABCEFGD and investigate the relationship between ureolytic activity expression of this clone in Escherichia coli and nickel ions. METHODS The target gene was cloned by polymerase chain reaction in 2 parts separately. Then, 2 plasmids were digested by specific restriction enzymes(More)
PURPOSE To observe the effect of anti-gingivitis IgY toothpaste in control of gingivitis and plaque. METHODS The study was a double-blind, randomized, parallel-controlled clinical trail with a total of 100 subjects who were divided into two groups, experimental group and control group. The subjects in experimental group used anti-gingivitis IgY toothpaste(More)
PURPOSE To prepare and identify polyclonal antibody of Streptococcus salivarius 57.I urease. METHODS The biggest structural subunit of Streptococcus salivarius 57.I urease, UreC, was obtained by gene clone, IPTG-induced expression, and purification through affinity chromatography. Anti-sera and polyclonal antibody were raised by immunizing rabbits with(More)
PURPOSE The aim of the study was to investigate the relationship between halitosis and Helicobacter pylori infection in stomach. METHODS Fifty subjects without periodontal diseases and systematic disease (exclude gastrointestinal diseases) were included. Infection of H.pylori was diagnosed by biopsy and (14)C-urea breath test. SPSS11.5 software package(More)
OBJECTIVE To clone Streptococcus salivarius (Ss) 57. I urease gene, which can express ureolytic activity in Escherichia coli (Ec) without adding extra nickel ions. METHODS Urease gene was cloned by polymerase chain reaction in three separate parts. The three separate plasmids were digested by specific restriction enzymes and ligated together. The(More)
PURPOSE To investigate the change of saliva flow rate and pH after chewing tea polyphenol gum. METHODS Saliva was collected before and after chewing tea polyphenol gum and the control gum. Both the salivary flow rate and pH value were measured and analyzed by one-way ANOVA and SNK. RESULTS The chewing gum significantly increased the flow rate as well as(More)
PURPOSE To investigate the knowledge, attitude and practice related to evidence-based dentistry among dental students, and to provide a reference for targeted evidence-based dentistry teaching and practice evidence-based dentistry in dental students. METHODS Dental students who attended the internship in Shanghai Ninth People's Hospital and Pudong(More)
PURPOSE The aim of the study was to evaluate the change of plaque pH after chewing 2 kinds of gums. METHODS The investigation consisted of 8 healthy subjects (aged 23-27 years, 4 males and 4 females) who refrained from toothbrushing for 24 hours before the test. The pH of dental plaque was measured using a Beetrode pH microelectrode before and after(More)