Dan O. Nelson

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The design of a tissue slice perfusion system is described, and examples are given showing the stability of this system for intracellular and extracellular recordings during changes in perfusion media. The stability of this system is attributed to several features. Mini-drips serve to cushion transient changes in flow rate when switching from one medium to(More)
With increasing availability of drugs for impotence and advanced reproductive technologies for the treatment of subfertility, more men are fathering children at advanced ages. We conducted a study of the chromosomal content of sperm of healthy men aged 24-57 years to (a) determine whether father's age was associated with increasing frequencies of aneuploid(More)
This study investigated the effect of chemical and electrical stimulation of the area postrema on renal sympathetic nerve activity (RSNA), arterial pressure, and heart rate in urethan-anesthetized rabbits. Electrical stimulation of the area postrema at 2, 5, 10, 20, 40, and 80 Hz using constant currents of 7.5, 15, and 30 microA (pulse duration = 0.3 ms,(More)
Single-unit, extracellular recordings were made from spontaneously active, thermosensitive neurons in the preoptic region of green sunfish acclimated to 25 degrees C. Activity of single cells was monitored during increases and decreases in local brain temperature over approximately a 10 degrees C range. Deep-body and skin temperatures were maintained at 25(More)
Local neural circuitry in the nucleus tractus solitarius (NTS) involved in cardiovascular control was studied by injecting nanoliter volumes of excitatory amino acids into the structure. Experiments were performed on urethane-anesthetized, artificially ventilated rats. Multibarrel micropipettes were used for pressure ejection of drugs or a dye for marking(More)
Compared to Wistar-Kyoto (WKY) normotensive control rats, spontaneously hypertensive (SHR) rats have significantly reduced brain weights (-10.6%) and brain volumes (-11.8%). Computerized morphometric analysis of soma cross-sectional areas of single neurons in 12 selected hypothalamic regions revealed significant differences between SHR and WKY animals.(More)
Base map compiled by Illinois State Geological Survey from digital data provided by the United States Geological Survey. Topography compiled by photogrammetric methods from aerial photographs taken 1986. PLSS current as of 1991. Planimetry derived from imagery taken 1998. State of Illinois make no guarantee, expressed or implied, regarding the correctness(More)