Damaris Carriero

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Mature erythrocytes, when removed from the circulation, exhibit severe disturbances of glycolytic flow, with accumulation not only of lactate, the ultimate product of glycolysis, but also of several upstream metabolic intermediates, primarily fructose-1,6-diphosphate, glyceraldehyde-3-phosphate, and dihydroxyacetone phosphate. This accumulation may be(More)
A systematic method was designed to screen a large population of patients with erythropoietic protoporphyria (EPP) for aberrant ferrochelatase RNA with skipped exons. The method utilizes the new junction sequence created by exon skipping as the probe to detect such RNA species. In 7 of 17 EPP families, an aberrant ferrochelatase RNA with one exon missing(More)
An aberrant ferrochelatase mRNA lacking exon 10 was found in a patient with erythropoietic protoporphyria (EPP). In her genomic DNA an A-->T transversion at position -3 of the donor site of intron 10 appeared to be responsible for the exon skipping. Both the patient and her sister were heterozygous for this mutation.
Buoyant density centrifugation on discontinuous gradients separates red blood cells (RBCs) according to age, as shown by radiolabelling experiments both in vitro and in vivo. Changes observed in these gradients reflect in vivo rates of decline. A progressive metabolic decline may render the RBC incapable of surviving stresses in the circulation. It was(More)
In spite of the declining prevalence of iron-deficiency anemia, a large proportion of low-income infants have "low-normal" (11-11.5 g/dL) and "low" (less than 11 g/dL) hemoglobin (Hgb) values. Because most of these infants are fed iron-fortified formulas, it was of interest whether additional iron supplementation would enhance Hgb values. A cohort of 334(More)
Hepatitis C infection is an important problem in inner city neighbourhoods, which suffer from multiple health disparities. Important factors in this population include alcoholism and substance abuse, mental illness and homelessness, which may be combined with mistrust, poor health literacy, limited access to healthcare and outright discrimination. Systemic(More)
Transanal Doppler-guided hemorrhoidal artery ligation with mucopexy and hemorrhoidopexy with the HemorPex System (HPS) (ANGIOLOGICA B.M. S.r.l., Pavia, Italy) are minimally invasive non-excisional methods available to treat symptomatic hemorrhoids not responding to more conservative treatment. The HPS makes possible mucopexy and the ligature of superior(More)
In children the levels of erythrocyte porphyrins (EP) increase exponentially with the blood lead (BPb) above a threshold of 18 μg/dl (P.N.A.S. 79: 3335, 1982). The EP levels are also elevated by iron deficiency. This study was directed to assess the response of normal and Fe deficient children to low levels of Pb. In addition to BPb and EP, serum ferritin(More)
In patients with sickle cell syndromes, EP levels may be markedly elevated. In 249 patients with sickle cell syndromes, we measured EP, ALAD, and ALAD after reactivation with dithiothreitol (ALADSH), in parallel with reticulocytes and pyruvate kinase (PK), two age-dependent parameters. EP, ALAD, ALADSH, reticulocytes and PK were much higher than normal in(More)
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