Dakshay Patel

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In the present study we have developed a simple, time, and cost effective in vivo rodent protocol to screen the susceptibility of a test compound for P-glycoprotein (P-gp) mediated efflux at the blood brain barrier (BBB) during early drug discovery. We used known P-gp substrates as test compounds (quinidine, digoxin, and talinolol) and elacridar (GF120918)(More)
High Titre, Ascaris specific, porcine reaginic antibody was induced by combining parasitic infestation and antigen challenge. The reagin was characterised as being heat labile at 56 degrees C, possessing long term skin sensitising activity, and as having physicochemical properties similar to IgE in other species. Porcine IgE was purified by conventional(More)
We have studied the attachment of immunomagnetic beads to different cells, with particular interest in cells that did not, as expected, appear to bind antibody-coated beads. Through the use of immunofluorescence and laser scanning confocal microscopy it was possible to demonstrate that beads can detach significant amounts of antigen from the surface of(More)
Oral immunization of the lactating sow with live Escherichia coli (O8:K88) stimulated an IgA anti-K88 antibody response in mammary secretions and significantly enhanced the capacity of whey to inhibit adhesion of the inoculum E. coli strain to isolate pig enterocytes. An increase in agglutinating anti-O8 activity was also evident in jilk and was associated(More)
The secretory and systemic antibody responses to oral dead Escherichia coli were examined in A strain mice. The best responses were obtained in mice dosed with 1 x 10(10) bacteria. Orally immunized animals showed an enhanced response to subsequent parenteral immunization with somatic antigens. This effect could be passively transferred with serum. In(More)
It has previously been demonstrated that cells can bind antibody-coated beads; this effect can be used to enhance the fractionation of cells using magnetic fields or by centrifugation on isopycnic, isotonic density gradients. As a general rule, the higher the expression of surface antigens the more beads bind to cells. However, we have also noted that other(More)
In the United Kingdom, the infection of people with Coxiella burnetii, the causative agent of Q fever, is of significant public health importance and is associated with contact with dairy cattle. An ELISA was developed for the detection of IgG antibodies against C burnetii in bulk tank milk, and in a survey of randomly selected samples from dairy herds in(More)
The fractionation of sub-populations of peripheral blood mononuclear cells has become an essential routine procedure and some of the main fractionation methods used today are immunomagnetic separations. We describe a less expensive method for the separation of subpopulations of mononuclear cells using density perturbation, which uses the binding of(More)
The limits of detection of IgG1 antibody in a standard mouse antiserum by a single antiglobulin enzyme-linked immunosorbent assay (ELISA) and 2 amplified systems, a double antiglobulin ELISA and a double antiglobulin radioimmunoassay (RIA), were compared in microtitration plates with the same antigen preparation and antisera. Compared with the single(More)
The crustacean intestine and hepatopancreas display a variety of solute transport mechanisms for transmembrane transfer of dietary contents from lumen to epithelial cytosol. An in vitro intestinal perfusion apparatus was used to characterize mucosal to serosoal (MS) and serosal to mucosal (SM) Zn(2+) -dependent (3)H-L-leucine transport by the intestine of(More)