Daisuke Seto

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A novel fluorimetric assay for dopamine using calcein blue (CB) complexed with Fe(2+) ion as a chemical sensor is described. The fluorescence arising from CB of the CB-Fe(2+) complex is quenched by the Fe(2+) ion. When dopamine is added to a solution of the CB-Fe(2+) complex, a dopamine-Fe(2+) complex is formed as the result of a ligand exchange reaction(More)
We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni(2+) and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni(2+) ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity(More)
A rapid and sensitive immunoassay for the determination of linear alkylbenzene sulfonates (LAS) is described. The method involves a sequential injection analysis (SIA) system equipped with a chemiluminescence detector and a neodymium magnet. Magnetic beads, to which an anti-LAS monoclonal antibody was immobilized, were used as a solid support in an(More)
A fluorescent probe, DPPEC (1,2-dipalmitoylglycerophosphorylethanolamine labeled with coumarin) was developed for detecting hydroxyl radical (*OH) in lipid membranes. The coumarin moiety contributes to the fluorescent detection of *OH and the phospholipids moiety gives a driving force to localize the probe in lipid membranes. DPPEC in liposomal membranes(More)
The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag.
A novel fluorescent probe composed of two moieties, Nile Red and an iminodiacetic acid-Ni(2+) complex, for the detection of histamine in living cells is described. The probe was successfully applied to visualizing histamine in RAW264 cells, representing the first demonstration of the imaging of histamine itself in living cells.
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