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This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and(More)
We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription(More)
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary(More)
—This paper discusses the design optimization of a wing for supersonic transport (SST) using a multiple-objective genetic algorithm (MOGA). Three objective functions are used to minimize the drag for supersonic cruise, the drag for transonic cruise, and the bending moment at the wing root for supersonic cruise. The wing shape is defined by 66 design(More)
This paper describes an application of Adaptive Range Multiobjective Genetic Algorithms (ARMOGAs) to aerodynamic wing optimization. The objectives are to minimize transonic and supersonic drag coefficients, as well as the bending and twisting moments of the wings for the supersonic airplane. A total of 72 design variables are categorized to describe the(More)
Recently, it has been proposed that novel methodologies are needed to re-evaluate apoptotic cell death, as studies of apoptosis have shown it to be a complex process. Since mitochondria are key regulators in cell death pathways, we developed a simultaneous 3-parameter flow cytometric analysis that incorporates the change in mitochondrial membrane potential(More)
Self-Organizing Maps (SOMs) have been used to visualize tradeoffs of Pareto solutions in the objective function space for engineering design obtained by Evolutionary Computation. Furthermore, based on the codebook vectors of cluster-averaged values of respective design variables obtained from the SOM, the design variable space is mapped onto another SOM.(More)
It is becoming increasingly clear that alternative splicing enables the complex development and homeostasis of higher organisms. To gain a better understanding of how splicing contributes to regulatory pathways, we have developed an alternative splicing library approach for the identification of alternatively spliced exons and their flanking regions by(More)