Daisuke Saito

Learn More
Preoptic magnocellular neurosecretory cells (NSCs) in the brain of rainbow trout show synchronization of periodic Ca(2+) pulses, patterns of which differ between vasotocin (VT) and isotocin (IT) neurons. To provide neuroanatomical bases of the synchronized periodic Ca(2+) pulses and their biological implications, we examined the organization of preoptic VT(More)
During early morphogenesis, tissue segregation is often accompanied by changes in cell shape. To understand how such coordination is regulated, somitogenesis was used as a model. When a somite forms in the anterior end of the presomitic mesoderm, an intersomitic boundary (gap) emerges, and it is rapidly followed by cell epithelialization at this border. It(More)
The autonomic nervous system, which includes the sympathetic neurons and adrenal medulla, originates from the neural crest. Combining avian blood vessel-specific gene manipulation and mouse genetics, we addressed a long-standing question of how neural crest cells (NCCs) generate sympathetic and medullary lineages during embryogenesis. We found that the(More)
We consider systems of differential equations which model complex regulatory networks by a graph structure of dependencies. We show that the concepts of informative nodes (Mochizuki; see [MoSa10]) and determining nodes (Foias and Temam; see [FoTe84]) coincide with the notion of feedback vertex sets from graph theory. As a result we can determine the(More)
Limb-type-specific expression of Tbx5/Tbx4 plays a key role in drawing distinction between a forelimb and a hindlimb. Here, we show insights into specification and determination during commitment of limb-type identity, in particular that median tissues regulate Tbx expressions. By using the RT-PCR technique on chick embryos, the onset of specific Tbx5/Tbx4(More)
The in ovo electroporation technique in chicken embryos has enabled investigators to uncover the functions of numerous developmental genes. In this technique, the ubiquitous promoter, CAGGS (CMV base), has often been used for overexpression experiments. However, if a given gene plays a role in multiple steps of development and if overexpression of this gene(More)
Blood vessels in the central nervous system supply a considerable amount of oxygen via intricate vascular networks. We studied how the initial vasculature of the spinal cord is formed in avian (chicken and quail) embryos. Vascular formation in the spinal cord starts by the ingression of intra-neural vascular plexus (INVP) from the peri-neural vascular(More)
This paper presents the first version of a speaker verification spoofing and anti-spoofing database, named SAS corpus. The corpus includes nine spoofing techniques, two of which are speech synthesis, and seven are voice conversion. We design two protocols, one for standard speaker verification evaluation, and the other for producing spoofing materials.(More)
Tetrapod limbs, forelimbs and hindlimbs, emerge as limb buds during development from appropriate positions along the rostro-caudal axis of the main body. In this study, tissue interactions by which rostro-caudal level-specific limb initiation is established were analyzed. The limb bud originates from the lateral plate located laterally to the paraxial(More)
This paper describes a novel approach to construct a mapping function between a given speaker pair using probability density functions (PDF) of matrix variate. In voice conversion studies, two important functions should be realized: 1) precise modeling of both the source and target feature spaces, and 2) construction of a proper transform function between(More)