Daisuke Kurose

Learn More
Most of the many Stemphylium species on record as plant pathogens in Japan have been identified by morphology. Using molecular phylogenetic analysis of four loci (rDNA-ITS, EF-1α, GPD, and vmaA-vpsA) combined, we re-examined the taxonomy of 31 Stemphylium strains that had been identified morphologically before or after their deposit in the NIAS Genebank,(More)
Although there has been much research on soil microbial communities in organic farming, little research has been reported on those in natural farming (no fertilizer use). Soil chemical properties and microbial and nematode communities in naturally (Orchard-N) and conventionally (Orchard-C) farmed apple orchards were compared over 3 years as a case study.(More)
Ralstonia solanacearum is the causal organism of bacterial wilt of more than 200 species representing 50 families of plants in tropical, subtropical, and warm temperate regions in the world. Traditionally classified into five races based on differences in host range, R. solanacearum has also been grouped into six biovars on the basis of biochemical(More)
Yellow spots on leaves of white lace flower, Ammi majus, were found in Chiba Prefecture, Japan, in October 2006. The symptoms later developed into large brown spots. Brown lesions were also observed on the lower stem, and stem rot followed. A fungus was frequently isolated from the lesions, and the symptoms were reproduced after artificial inoculation. The(More)
A preemergence damping-off of tobacco (Nicotiana tabacum L.) was found in Fukuoka, Japan, in 2009. A fungus repeatedly isolated from the black-rotted seeds reproduced the symptoms in tobacco seeds, and the preemergence damping-off developed after flower and soil inoculation with the fungus. Brown spots and blight developed on leaves of tobacco seedlings(More)
Leaf spots on spinach were found in three greenhouses in Hokkaido and Iwate, Japan, in 2011–2012. Three isolates obtained from the lesions were classified into Stemphylium sp. Subgroups C2 and E3, based on morphology and molecular analyses. We compared the three isolates with two reference isolates belonging to Subgroup C2 from Welsh onion and leek in terms(More)
From 2007 to 2013, a disease of Welsh onion, causing leaf sheath rot and concomitant death of outer leaves was found in 20 fields in Hokkaido, Japan. We obtained 20 Rhizoctonia isolates from diseased tissues and identified them based on the number of nuclei, hyphal fusion reactions, and molecular techniques using specific PCR primers and sequence of the(More)