Daigo Kobayashi

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BACKGROUND Patched 1 (Ptc1) is a polytopic receptor protein that is essential for growth and differentiation. Its extracellular domains accept its ligand, Sonic Hedgehog, while the function of its C-terminal intracellular domain is largely obscure. PRINCIPAL FINDINGS In this study, we stably expressed human Ptc1 protein in HeLa cells and found that it is(More)
Selective packaging of plasmid DNA (pDNA) into folded rod or collapsed sphere structures in polyplex micelles was demonstrated by modulating the PLys segment length of poly(ethylene glycol)-block- poly(L-lysine) (PEG-PLys) block catiomers used for micelle formation. The two basic packaging structures correlated well to the integrity of double-stranded DNA(More)
We successfully dissolved wet and saliferous microalgae (WSM) in polar ionic liquids (ILs) under mild conditions. The Kamlet-Taft parameters, especially β for the ILs, were good predictors of the ability to dissolve WSM. 1-Ethyl-3-methylimidazolium methylphosphate ([C2mim][MeO(H)PO(2)]) was the IL that best dissolved WSM without heating. WSM (containing 95(More)
We present a new user interface for the virtual sculpting system. In our system, the user operates a chisel with a pressure sensitive pen to carve a workpiece in the virtual space. The user can control the depth of carving and the angle of the virtual chisel to the surface of the workpiece dynamically like the real wood carving.
Highly regulated folding of plasmid DNA (pDNA) through polyion complexation with the synthetic block catiomer, poly(ethylene glycol)-block-poly(L-lysine) (PEG-PLys), was found to occur in such a way that rod structures are formed with a quantized length of 1/2(n + 1) of the original pDNA length folding by n times. The folding process of pDNA was elucidated(More)
In this paper, we present an interactive CG creating system with a pressure sensitive pen and a tablet. This system is based on the virtual sculpting and printing method which are simulations of the real sculpting and woodblock printing. In the virtual sculpting process, the user operates virtual chisels with the pen to sculpt a virtual wooden object which(More)
Here, we proposed a simple recovery process for poly(3-hydroxybutyrate) (PHB) accumulated in cyanobacteria by using ionic liquids (ILs), which dissolve cyanobacteria but not PHB. First, we investigated the effects of IL polarity on hydrogen-bonding receipt ability (β value) and hydrogen-bonding donating ability (α value) and evaluated the subsequent(More)