Dafne A. Oliveira

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AMA-1 of Plasmodium falciparum is a promising candidate antigen in malaria vaccine development. In this study, we have mapped the immunodominant T-cell determinants in this antigen by using synthetic peptides. From the amphipathic scores, 17 putative T-cell determinants were identified. Nine of the 17 peptides complementary to the putative T-cell(More)
In a blind field evaluation of a nonisotopic liquid hybridization assay for detection of malaria parasites, 100 blood samples were tested from an area in which malaria is endemic; light microscopy was used as the reference test. Sensitivity, specificity, and positive and negative predictive values of the hybridization assay were 100%. One sample that was(More)
Maize (Zea mays L.) is a crop of global importance, but prone to contamination by aflatoxins produced by fungi in the genus Aspergillus. The development of resistant germplasm and the identification of genes contributing to resistance would aid in the reduction of the problem with a minimal need for intervention by farmers. Chitinolytic enzymes respond to(More)
In the present study, we describe a polymerase chain reaction (PCR)-based enzyme-linked immunosorbent assay for the detection of malaria infection. The target region of the 18S ribosomal DNA is amplified by a PCR using an 18S rRNA, genus-specific, biotinylated (5') and an unlabeled primer (3') pair. The detection probes are digoxigenin-labeled DNA(More)
The growth rate and the protein level of two swine cell sublines, one of them with a diploid and the other with a tetraploied level of chromosomes, were studied during 96 hours at 37--38 degrees C. Both of them showed similar growth rates, although the cell number of the diploid cell subline had been higher than the tetraploid cell subline on the first(More)
Experiment to study the evolution of infestations of Corcyra cephalonica in peeled peanuts, grain corn, wheat flour and grain coffee, were carried out in Santos and in São Paulo (State of São Paulo, Brazil). In the experimental conditions, more emergence resulted from peanuts followed by grain corn and third by wheat flour; a very small number of moths(More)
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