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Confocal laser scanning microscopy (CLSM) and whole-cell patch-clamp were used to investigate the role of Ca2+ influx in maintaining the cytosolic Ca2+ concentration ([Ca2+]c) and the features of the Ca2+ influx pathway in germinating pollen grains of Lilium davidii D. [Ca2+]c decreased when Ca2+ influx was inhibited by EGTA or Ca2+ channel blockers. A(More)
Apoptosis is the predominant form of cell death observed in a variety of physiological and pathological conditions such as cancer involution, insect metamorphosis, the development of the immune and nervous systems, and embryogenesis. The typical nuclear changes taking place in apoptotic cells include extensive condensation of chromatin and internucleosomal(More)
To establish objectively the involvement of neurons in the medulla oblongata in patients with amyotrophic lateral sclerosis (ALS), ribonucleic acid (RNA) content was determined in neurons of the hypoglossal nucleus and the nucleus ambiguus. Neurons from those two nuclei showed a significant loss of RNA content in patients with ALS; only 57% and 38% of the(More)
The distribution of integrin-like proteins in the pollen tube was examined by immunofluorescent labeling and western blotting techniques using antibodies against human placenta integrin vitronectin receptor (VnR), and alpha(v), beta3 and beta1 integrin subunits. Pseudocolor-coded confocal images showed intense immunostaining within 10 and 5 microm of the(More)
The phosphatidylinositol-specific phospholipase C (PI-PLC) activity is detected in purified Lilium pollen protoplasts. Two PI-PLC full length cDNAs, LdPLC1 and LdPLC2, were isolated from pollen of Lilium daviddi. The amino acid sequences for the two PI-PLCs deduced from the two cDNA sequences contain X, Y catalytic motifs and C2 domains. Blast analysis(More)
We demonstrate a hydrostatic pressure-induced hard-to-soft transition of an isolated single wall carbon nanotube, using classical and ab initio constant-pressure molecular-dynamics simulations and continuum elastic theory analysis. At low pressure, the carbon tube is hard. Above a critical pressure, the tube becomes much softer with a decrease of bulk(More)
OBJECTIVE To describe a unique case of acute asymmetrical myopathy following high-dose intravenous use of corticosteroids that initially mimicked a stroke and then evolved into a picture suggestive of myelopathy. DESIGN Case report. SETTING Tertiary hospital. PATIENTS A 71-year-old woman treated with high-dose steroids for an acute asthmatic(More)
An antisense oligonucleotide (54 mer) from the mRNA to the midsize neurofilament protein (NFM) was labeled with 35S on the 3' end and purified by polyacrylamide gel electrophoresis (PAGE). In situ hybridization was performed on sections from medulla oblongata of patients with amyotrophic lateral sclerosis (ALS). The slides were dipped in photographic(More)
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