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Somatic cell nuclear transfer (SCNT) has been performed extensively in fish since the 1960s with a generally low efficiency of approximately 1%. Little is known about somatic nuclear reprogramming in fish. Here, we utilized the zebrafish as a model to study reprogramming events of nuclei from tail, liver and kidney cells by SCNT. We produced a total of(More)
The transgenic promoter directly affects the activity of transgenic gene. Choice of terminator has been proved to affect the activity of transgenic gene. Since the relevant theoretical research is rare, using the terminator of transgenic gene or the promoter gene to construct the transgenic vector has not come to the conclusion. In order to construct a(More)
High length and nucleotide polymorphisms in intron2 of GH I gene were detected in 162 individuals,which were from seven wild crucian carp colonies, two goldfish colonies and one Fangzheng crucian carp colony. Using denaturating polyareylamide gel electrophoresis (DPAGE) and single-strand conformation polymorphism (SSCP), seven length variants and 15(More)
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