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Murine spleen cells were depleted of specific B-cell subpopulations bearing different immunoglobulin isotypes by means of complement-mediated cytolysis after treatment with antisera specific for micron- and gamma-chains. The functional effect of this depletion was measured by assaying both the primary and secondary plaque-forming cell responses of the(More)
The induction of antibody secretion by B cells requires T-cell-derived factors1-5. Such factors have been described1,2,6-12 but the precise relationship among these various factors is not clear, and it has been difficult to demonstrate that these factors act directly on the B cell and do not exert their effect via T cells or macrophages. In this report we(More)
Analysis of mu-specific mRNA in the B cell tumor line, BCL1, shows that the cells contain predominantly mRNA for mu chain of membrane-bound immunoglobulin M (IgM) (2.7 kb, mu m mRNA). Stimulation of the cells to Ig secretion by lipopolysaccharide (LPS) results in a 6-12 fold increase in amount of mRNA for the mu chain of secreted IgM (2.4 kb mu s mRNA). The(More)
The majority of adult B lymphocytes in the mouse bear two immunoglobulin isotypes, IgM and IgD (mu(+)delta(+) cells) (1). A small population of IgM-bearing cells lacks, or expresses very low levels of IgD (mu- predominant [mup] cells) (1). These cells are believed to constitute a less mature subset of B cells analogous to neonatal B cells (2). Based on the(More)
Intestinal motility and barriers are often impaired due to intestinal congestion during liver transplantation. Intestinal bacteria and enterogenous endotoxins enter into the blood stream or lymphatic system and translocate to other organs, which can result in postoperative multi-organ dysfunction (MODF) and systemic inflammatory reaction syndrome (SIRS)(More)
Biochemical investigations have demonstrated that nitric oxide synthase (NOS) is distributed widely in the olfactory system. However, little is known about the action of NO at the synaptic level on identified neurons in local circuits that process chemosensory signals. Here, using whole-cell recordings, the effect of NO on cholinergic synaptic input to(More)
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