DARKEN A. MILLER

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Human and higher primate chromosomes have been compared by general and regional banding methods, including hybridization in situ. The general banding patterns of the chromosomes of gorilla, chimpanzee, and orangutan, but not gibbon, are similar to those of the human. Preliminary results show that chromosomes with similar banding patterns in different(More)
Frog skins mounted between chambers without compression, with a liquid insulator and a tissue adhesive, were studied in vitro. Since voltage and electrical resistance per unit area were unchanged by a tenfold decrease in area of skin studied, it is concluded that these mounting techniques do not produce edge damage and consequently provide a means for study(More)
C19 steroids are converted to estrogens in a number of human tissues by the aromatase enzyme complex, which consists of aromatase cytochrome P450 (P450arom; product of the CYP19 gene) and NADPH-cytochrome P450 reductase. Aromatase activity has been previously demonstrated in endometrial tumors. In the present study, we investigated CYP19 gene expression and(More)
All-trans-retinoic acid is metabolized in vitro to a biologically active metabolite, retinoyl-beta-glucuronide. We have studied the synthesis of this metabolite in vitro. The identity of the product was established by cochromatography on reverse-phase high-performance liquid chromatography, beta-D-glucuronidase hydrolysis, and fast atom bombardment and(More)
Oleic acid (OA) administered to experimental animals increases pulmonary vascular permeability and produces a condition that pathophysiologically resembles adult respiratory distress syndrome (ARDS) in humans. The present study examined the sequence of cardiorespiratory changes after OA infusion and their similarity to ARDS. After a baseline period,(More)
Two synthetic retinoids were examined for their ability to support growth in male vitamin A-deficient rats. One of the compounds, (E)-4-[2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1 -propenyl]-benzoic acid (TTNPB), was found to be highly effective; it was 35-fold more active than all-trans-retinoic acid. Thus, the in vivo results were in(More)