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Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology
TLDR
A novel method based on strain-dependent hybridization patterns of in vitro-amplified DNA with multiple spacer oligonucleotides was found to differentiate M. bovis from M. tuberculosis, a distinction which is often difficult to make by traditional methods.
Proposal for Standardization of Optimized Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat Typing of Mycobacterium tuberculosis
TLDR
A discriminatory subset of 15 loci with the highest evolutionary rates was defined that concentrated 96% of the total resolution obtained with the full 24-locus set, and its predictive value for evaluating M. tuberculosis transmission was found to be equal to that of IS6110 restriction fragment length polymorphism typing.
Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology
TLDR
The results suggests the existence of fine geographical genetic clines within MTC populations, that could mirror the passed and present Homo sapiens sapiens demographical and mycobacterial co-evolutionary history whose structure could be further reconstructed and modelled, thereby providing a large-scale conceptual framework of the global TB Epidemiologic Network.
A new evolutionary scenario for the Mycobacterium tuberculosis complex
TLDR
The distribution of 20 variable regions resulting from insertion-deletion events in the genomes of the tubercle bacilli has been evaluated and contradict the often-presented hypothesis that M. tuberculosis, the etiological agent of human tuberculosis evolved from M. bovis, the agent of bovine disease.
Comparison of Methods Based on Different Molecular Epidemiological Markers for Typing of Mycobacterium tuberculosis Complex Strains: Interlaboratory Study of Discriminatory Power and Reproducibility
TLDR
Strain differentiation by IS6110 RFLP or mixed-linker PCR are the methods of choice for epidemiological investigations, indicating a clonal population structure of M. tuberculosis strains.
Automated High-Throughput Genotyping for Study of Global Epidemiology of Mycobacterium tuberculosis Based on Mycobacterial Interspersed Repetitive Units
ABSTRACT Large-scale genotyping of Mycobacterium tuberculosisis especially challenging, as the current typing methods are labor-intensive and the results are difficult to compare among laboratories.
Predominance of a single genotype of Mycobacterium tuberculosis in countries of east Asia
TLDR
Analysis of the population structure of Mycobacterium tuberculosis strains from the People's Republic of China showed that the vast majority belong to a genetically closely related group, which is designated the "Beijing family" of M. tuberculosis strains, indicating that strains of the Beijing family recently expanded from a single ancestor which had a selective advantage.
Novel Genetic Polymorphisms That Further Delineate the Phylogeny of the Mycobacterium tuberculosis Complex
TLDR
Evaluating known MTC phylogenetic markers in a larger collection of tubercle bacilli and evaluating additional recently reported MTC species-specific and interspecific polymorphisms add to the genetic definitions of several MTC organisms as well as fine-tune current models for the evolutionary history of the MTC.
PCR-Based Method To Differentiate the Subspecies of the Mycobacterium tuberculosis Complex on the Basis of Genomic Deletions
TLDR
This MtbC PCR typing panel provides an advanced approach to determine the subspecies of MtbB isolates and to differentiate them from clinically important MOTT species and may be applied for practical clinical and epidemiological use.
A novel pathogenic taxon of the Mycobacterium tuberculosis complex, Canetti: characterization of an exceptional isolate from Africa.
TLDR
This study supports the idea that the established members within the M. tuberculosis complex and the newly described Canetti grouping should be regarded as a single species, which likely will be designated "M. tuberculosis".
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