D. Zahradka

Publications50
h-index11
Citations277
Highly Influential Citations15
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Roles of ExoI and SbcCD nucleases in "reckless" DNA degradation in recA mutants of Escherichia coli.
Exponentially growing recA mutant cells of Escherichia coli display pronounced DNA degradation that starts at the sites of DNA damage and depends on RecBCD nuclease (ExoV) activity. As a consequence… Expand
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Lethality of bypass polymerases in Escherichia coli cells with a defective clamp loader complex of DNA polymerase III
Escherichia coli DNA polymerase III (Pol III) is one of the best studied replicative DNA polymerases. Here we report the properties of an E. coli mutant that lacks one of the subunits of the Pol III… Expand
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sbcB15 And DeltasbcB mutations activate two types of recf recombination pathways in Escherichia coli.
Escherichia coli cells with mutations in recBC genes are defective for the main RecBCD pathway of recombination and have severe reductions in conjugational and transductional recombination, as well… Expand
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RecA protein assures fidelity of DNA repair and genome stability in Deinococcus radiodurans.
Deinococcus radiodurans is one of the most radiation-resistant organisms known. It can repair hundreds of radiation-induced double-strand DNA breaks without loss of viability. Genome reassembly in… Expand
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Exonuclease VII is involved in "reckless" DNA degradation in UV-irradiated Escherichia coli.
The recA mutants of Escherichia coli exhibit an abnormal DNA degradation that starts at sites of double-strand DNA breaks (DSBs), and is mediated by RecBCD exonuclease (ExoV). This "reckless" DNA… Expand
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The RuvABC resolvase is indispensable for recombinational repair in sbcB15 mutants of Escherichia coli.
The RuvABC proteins of Escherichia coli play an important role in the processing of Holliday junctions during homologous recombination and recombinational repair. Mutations in the ruv genes have a… Expand
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Krypton tagging velocimetry in a turbulent Mach 2.7 boundary layer
The krypton tagging velocimetry (KTV) technique is applied to the turbulent boundary layer on the wall of the “Mach 3 Calibration Tunnel” at Arnold Engineering Development Complex (AEDC) White Oak.… Expand
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Could LogP be a principal determinant of biological activity in 18-crown-6 ethers? Synthesis of biologically active adamantane-substituted diaza-crowns.
18-crown-6 ethers are known to exert their biological activity by transporting K(+) ions across cell membranes. Using non-linear Support Vector Machines regression, we searched for structural… Expand
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RecBCD enzyme overproduction impairs DNA repair and homologous recombination in Escherichia coli.
The Escherichia coli RecBCD enzyme is a powerful helicase and nuclease that processes DNA molecules containing blunt double-strand DNA end. Mutants deprived of RecBCD enzyme functions are extremely… Expand
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RecF recombination pathway in Escherichia coli cells lacking RecQ, UvrD and HelD helicases.
In recBCD sbcB sbcC(D) mutants of Escherichia coli homologous recombination proceeds via RecF pathway, which is thought to require RecQ, UvrD and HelD helicases at its initial stage. It was… Expand
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