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Small-molecule inhibitors of microrna miR-21 function.
MicroRNAs (miRNAs) have recently emerged as an important class of gene regulators, and their misregulation has been linked to a variety of cancers. Small molecule inhibitors of miRNAs would beExpand
Small molecule modifiers of microRNA miR-122 function for the treatment of hepatitis C virus infection and hepatocellular carcinoma.
MicroRNAs are a recently discovered new class of important endogenous regulators of gene function. Aberrant regulation of microRNAs has been linked to various human diseases, most importantly cancer.Expand
Photochemical activation of protein expression in bacterial cells.
Small-molecule-inducible gene-expression systems are available in a variety of cell types including bacterial cells, yeast cells, and mammalian cells. They have been used for conditional proteinExpand
Photochemical control of biological processes.
Photochemical regulation of biological processes offers a high level of control to study intracellular mechanisms with unprecedented spatial and temporal resolution. This report summarizes theExpand
An evolved aminoacyl-tRNA synthetase with atypical polysubstrate specificity.
We have employed a rapid fluorescence-based screen to assess the polyspecificity of several aminoacyl-tRNA synthetases (aaRSs) against an array of unnatural amino acids. We discovered that aExpand
Microwave activation of enzymatic catalysis.
Microwave irradiation can be used to regulate biocatalysis. Herein, the utilization of hyperthermophilic enzymes in a microwave reactor is reported. While these enzymes are inactive at lowExpand
Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP.
We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesionExpand
Evolution of cyclic peptide protease inhibitors
We report a bacterial system for the evolution of cyclic peptides that makes use of an expanded set of amino acid building blocks. Orthogonal aminoacyl-tRNA synthetase/tRNACUA pairs, together with aExpand
Incorporation of fluorotyrosines into ribonucleotide reductase using an evolved, polyspecific aminoacyl-tRNA synthetase.
Tyrosyl radicals (Y·s) are prevalent in biological catalysis and are formed under physiological conditions by the coupled loss of both a proton and an electron. Fluorotyrosines (F(n)Ys, n = 1-4) areExpand
Site-specific incorporation of fluorotyrosines into proteins in Escherichia coli by photochemical disguise.
Fluorinated analogues of tyrosine can be used to manipulate the electronic environments of protein active sites. The ability to selectively mutate tyrosine residues to fluorotyrosines is limited,Expand
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