A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite.
A method for simultaneous evaluation of nitrate and nitrite concentrations in a microtiter plate format is developed and S-Nitrosothiols and L-arginine derivatives were found to be potential interfering agents.
Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide.
Methods for detection of reactive metabolites of oxygen and nitrogen: in vitro and in vivo considerations.
- M. Tarpey, D. Wink, M. Grisham
- Biology, ChemistryAmerican Journal of Physiology. Regulatory…
- 1 March 2004
This review describes approaches to the direct and indirect measurement of different reactive metabolites of oxygen and nitrogen and particular attention to a method's applicability for in vivo determinations will be addressed.
The chemical biology of nitric oxide: implications in cellular signaling.
Complexes of .NO with nucleophiles as agents for the controlled biological release of nitric oxide. Vasorelaxant effects.
Vasorelaxant potency (expressed as 1/EC50) was strongly correlated with the quantity of .NO calculated from the physicochemical data to be released in the interval required to achieve maximum relaxation at the EC50 doses, suggesting that such nucleophile/.NO adducts might generally be useful as vehicles for the nonenzymatic generation of nitric oxide, in predictable amounts and at predictable rates, for biological purposes.
New nitric oxide-releasing zwitterions derived from polyamines
The reaction of nitric oxide (NO) with polyamines has been studied, resulting in the discovery of a new type of NO-releasing compound having the structure RN[N(O)NO][sup [minus]](CH[sub 2])[sub…
"NONOates" (1-substituted diazen-1-ium-1,2-diolates) as nitric oxide donors: convenient nitric oxide dosage forms.
DNA deaminating ability and genotoxicity of nitric oxide and its progenitors.
Nitric oxide (NO), a multifaceted bioregulatory agent and an environmental pollutant, can also cause genomic alterations that may contribute to the incidence of deamination-related genetic disease and cancer.
Nitric oxide and redox mechanisms in the immune response
The chemistry of NO and ROS in the context of antipathogen activity and immune regulation is discussed and similarities and differences between murine and human production of these intermediates are discussed.