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Roles of Trp31 in High Membrane Binding and Proinflammatory Activity of Human Group V Phospholipase A2 *
Group V phospholipase A2 is a recently discovered secretory phospholipase A2(PLA2) that has been shown to be involved in eicosanoid formation in inflammatory cells, such as macrophages and mastExpand
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A continuous fluorescence displacement assay for the measurement of phospholipase A2 and other lipases that release long-chain fatty acids.
  • D. Wilton
  • Chemistry, Medicine
  • The Biochemical journal
  • 1 March 1990
1. A new continuous fluorescence assay for phospholipase A2 is described which involves the displacement of the highly fluorescent fatty-acid probe 11-(dansylamino)undecanoic acid from rat liverExpand
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The fatty acid analogue 11-(dansylamino)undecanoic acid is a fluorescent probe for the bilirubin-binding sites of albumin and not for the high-affinity fatty acid-binding sites.
  • D. Wilton
  • Chemistry, Medicine
  • The Biochemical journal
  • 15 August 1990
1. The fluorescent fatty acid probe 11-(dansylamino)undecanoic acid (DAUDA) binds with high affinity to bovine and human serum albumin (BSA and HSA) at three sites. 2. The Kd of the primary bindingExpand
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Bacterial cell membrane hydrolysis by secreted phospholipases A(2): a major physiological role of human group IIa sPLA(2) involving both bacterial cell wall penetration and interfacial catalysis.
The ability of human group IIa secreted phospholipase A(2) (human sPLA(2)) to hydrolyse the phospholipid membrane of whole cell suspensions of Gram-positive bacteria is demonstrated in real timeExpand
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The stereochemistry of hydrogen transfer from reduced nicotinamide-adenine dinucleotide phosphate in the reduction of ethylenic linkages during cholesterol biosynthesis.
It is shown that during the saturation of steroid carbon-carbon double bonds at Delta(24,25) and Delta(14,15) the ;hydride ion' originates from the 4B side of the NADPH.
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Combined effects of sphingomyelin and cholesterol on the hydrolysis of emulsion particle triolein by lipoprotein lipase.
Sphingomyelin (SM) is one of the major lipids in lipoproteins. However, its function in lipoprotein metabolism is unknown. In an attempt to understand the role that this lipid plays in modulation ofExpand
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A continuous fluorescence-displacement assay for triacylglycerol lipase and phospholipase C that also allows the measurement of acylglycerols.
  • D. Wilton
  • Biology, Medicine
  • The Biochemical journal
  • 15 May 1991
A new continuous fluorescence-displacement assay for enzymes that release long-chain fatty acids [Wilton (1990) Biochem. J. 266, 435-439] is described in detail for pig pancreatic triacylglycerolExpand
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An apparent lack of stereospecificity in the reaction catalysed by deoxyribose 5-phosphate aldolase due to methyl-group rotation and enolization before product release.
In the reaction catalysed by deoxyribose 5-phosphate aldolase (2-deoxy-D-ribose 5-phosphate acetaldehyde-lyase, EC from Salmonella typhimurium, almost complete equilibration of theExpand
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A detailed investigation of the properties of lactate dehydrogenase in which the 'Essential' cysteine-165 is modified by thioalkylation.
The reaction of pig heart lactate dehydrogenase with methyl methanethiosulphonate resulted in the modification of one thiol group per protomer, and this was located at cysteine-165 in the enzymeExpand
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4,4'-dimethylcholesta-7,9,14-trienol is an intermediate in the demethylation of dihydroagnosterol.
1. 4,4'-Dimethylcholesta-7,9,14-trienol is an intermediate in the metabolism of dihydroagnosterol to cholesterol by rat liver homogenate. 2. This triene is reduced by a rat liver microsomalExpand
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