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Domain structure of an N-ethylmaleimide-sensitive fusion protein involved in vesicular transport.
Sequence analysis and proteolytic dissection reveal that NSF contains two tandem "ATP domains," each containing the consensus sequence for the binding of nucleotide. Expand
pH-dependent binding of KDEL to its receptor in vitro.
The results raise the possibility that erd2p-mediated sorting of lumenal endoplasmic reticulum proteins is facilitated by the pH differences between compartments of the secretory pathway. Expand
Mutational analysis of the human KDEL receptor: distinct structural requirements for Golgi retention, ligand binding and retrograde transport.
The KDEL receptor is a seven‐transmembrane‐domain protein that is responsible for the retrieval of endoplasmic reticulum (ER) proteins from the Golgi complex. It is a temporary resident of the GolgiExpand
A multisubunit particle implicated in membrane fusion
It is shown that NSF only binds to SNAPs in the presence of the integral receptor, resulting in the formation of a multisubunit protein complex with a sedimentation coefficient of 20S, and suggested how cycles of complex assembly and disassembly could help confer specificity to the generalized NSF-dependent fusion apparatus. Expand
Study of herpes simplex virus maturation during a synchronous wave of assembly
By assaying synchronized scaffold cleavage, DNA packaging, and acquisition of infectivity, this system should prove a generally useful tool for the molecular dissection of many late events in HSV-1 biogenesis. Expand
Mutations in the cytoplasmic tail of herpes simplex virus glycoprotein H suppress cell fusion by a syncytial strain
It is demonstrated that changes in the non-syn locus UL22-gH can reverse the syncytial phenotype of herpes simplex virus type 1 glycoprotein gH and the different requirements for cell-cell and cell-envelope fusion are emphasized. Expand
Mechanisms and pathology of monocrotaline pulmonary toxicity.
Experimental evidence supporting hypotheses that MCT is activated to a reactive metabolite in the liver and is then transported by red blood cells to the lung, where it initiates endothelial injury is presented. Expand
Infection kinetics, prostacyclin release and cytokine-mediated modulation of the mechanism of cell death during bluetongue virus infection of cultured ovine and bovine pulmonary artery and lung
Results of these in vitro studies are consistent with the marked pulmonary oedema and microvascular thrombosis that characterize bluetongue disease of sheep but which rarely, if ever, occur in BTV-infected cattle. Expand
An endoplasmic reticulum-retained herpes simplex virus glycoprotein H is absent from secreted virions: evidence for reenvelopment during egress
The hypothesis that the herpesvirus then undergoes de-envelopment, followed by reen envelopment at membranes outside the endoplasmic reticulum (ER), is tested by constructing a recombinant virus in which the expression of an essential glycoprotein is restricted to the inner nuclear membrane-ER by means of the ER retention motif, KKXX. Expand
Extranuclear gene expression in yeast: evidence for a plasmid-encoded RNA polymerase of unique structure.
Sequencing of plasmid k2, which is thought to encode factors necessary for the maintenance or expression of k1, reveals an open reading frame predicted to encode a 974 amino acid polypeptide with homology to several DNA-directed RNA polymerases, and it is suggested that this is a component of a novel plasmids-specific extranuclear gene expression system. Expand