• Publications
  • Influence
The Continuing Challenges of Leprosy
SUMMARY Leprosy is best understood as two conjoined diseases. The first is a chronic mycobacterial infection that elicits an extraordinary range of cellular immune responses in humans. The second isExpand
  • 665
  • 33
  • PDF
Characterization of rifampin-resistance in pathogenic mycobacteria.
The emergence of rifampin-resistant strains of pathogenic mycobacteria has threatened the usefulness of this drug in treating mycobacterial diseases. Critical to the treatment of individuals infectedExpand
  • 282
  • 12
PCR identification of Mycobacterium bovis BCG.
The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and otherExpand
  • 168
  • 10
Contribution of rpoB Mutations to Development of Rifamycin Cross-Resistance in Mycobacterium tuberculosis
ABSTRACT The contributions of 23 insertion, deletion, or missense mutations within an 81-bp fragment of rpoB, the gene encoding the β-subunit of the DNA-dependent RNA polymerase of MycobacteriumExpand
  • 226
  • 9
  • PDF
Direct genotypic detection of Mycobacterium tuberculosis rifampin resistance in clinical specimens by using single-tube heminested PCR.
Recent analysis of the gene encoding the beta subunit of Mycobacterium tuberculosis RNA polymerase (rpoB) has demonstrated a small region that harbors the mutations most frequently associated withExpand
  • 86
  • 9
Characterization of the catalase-peroxidase gene (katG) and inhA locus in isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: restricted array of
The catalase-peroxidase gene (katG) and a two-gene locus (inhA) containing mutations associated with resistance to isoniazid in Mycobacterium tuberculosis were sequenced in 34 resistant and 12Expand
  • 290
  • 7
Phenotypic and Genotypic Characterization ofMycobacterium africanum Isolates from West Africa
ABSTRACT The Mycobacterium tuberculosis complex includesM. tuberculosis, M. bovis, M. africanum, and M. microti. Most clinical isolates areM. tuberculosis or M. bovis. These species can beExpand
  • 67
  • 7
  • PDF
Molecular Determination of Mycobacterium leprae Viability by Use of Real-Time PCR
ABSTRACT Mycobacterium leprae, the etiological agent of leprosy, is noncultivable on axenic media. Therefore, the viability of M. leprae for clinical or experimental applications is often unknown. ToExpand
  • 96
  • 6
  • PDF
Dihydropteroate Synthase of Mycobacterium leprae and Dapsone Resistance
ABSTRACT Two Mycobacterium leprae genes, folP1 andfolP2, encoding putative dihydropteroate synthases (DHPS), were studied for enzymatic activity and for the presence of mutations associated withExpand
  • 81
  • 5
  • PDF
Rapid identification of a point mutation of the Mycobacterium tuberculosis catalase-peroxidase (katG) gene associated with isoniazid resistance.
The complete catalase-peroxidase (katG) gene DNA sequence was determined for 15 strains of Mycobacterium tuberculosis with a wide range of susceptibility to isoniazid. Five of 9 strains withExpand
  • 106
  • 4