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Karyotyping human chromosomes by combinatorial multi-fluor FISH
TLDR
The data suggest that multiplex-fluorescence in situ hybridization (M-FISH) could have wide clinical utility and complement standard cytogenetics, particularly for the characterization of complex karyotypes. Expand
Fusion of the TEL gene on 12p13 to the AML1 gene on 21q22 in acute lymphoblastic leukemia.
TLDR
The cloning of a t(12;21) translocation breakpoint involving 12p13 and 21q22 in two cases of childhood pre-B acute lymphoblastic leukemia shows that TEL, previously shown to be fused to the platelet-derived growth factor receptor beta in chronic myelomonocytic leukemia, can be implicated in the pathogenesis of leukemia through its fusion to either a receptor tyrosine kinase or a transcription factor. Expand
Mutation detection and single-molecule counting using isothermal rolling-circle amplification
TLDR
Rolling-circle amplification (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear or geometric kinetics under isothermal conditions, making it particularly amenable for the analysis of rare somatic mutations. Expand
Mutation in the DNA mismatch repair gene homologue hMLH 1 is associated with hereditary non-polyposis colon cancer
TLDR
It is reported that a human gene encoding a protein, hMLHl (human MutL homologue), homologous to the bacterial DNA mismatch repair protein MutL, is located on human chromosome 3p21.3-23. Expand
Delineation of DNA replication time zones by fluorescence in situ hybridization.
TLDR
Fluorescence in situ hybridization has been used to visualize specific genomic DNA sequences in interphase nuclei and it has been possible to map the replication timing topography of the DNA within and flanking the cystic fibrosis gene locus on chromosome 7. Expand
Nuclear foci of mammalian Rad51 recombination protein in somatic cells after DNA damage and its localization in synaptonemal complexes.
TLDR
It is concluded that the mammalian proteins homologous to yeast Rad51 are involved in repair of DNA damage and recombinational repair during meiosis. Expand
Immunological method for mapping genes on Drosophila polytene chromosomes.
TLDR
This immunological approach offers four advantages over conventional autoradiographic procedures for detecting in situ hybrids: the time required to determine the site of hybridization is decreased markedly, the biotin-labeled probes are chemically stable and give reproducible results for many months, and the resolving power is equal to and often greater than that achievedautoradiographically. Expand
High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones.
TLDR
The results demonstrate the feasibility of rapidly producing high-resolution maps of human chromosomes by in situ hybridization and show that by hybridizing three or more cosmids simultaneously, gene order on the chromosome could be established unequivocally. Expand
Primary Structure, Functional Expression, and Chromosomal Localization of the Bumetanide-sensitive Na-K-Cl Cotransporter in Human Colon (*)
TLDR
The human colonic cotransporter gene was localized to human chromosome 5 at position 5q23 and stably transfected cells exhibited a 15-fold greater bumetanide-sensitive Rb influx than control cells, and this flux required external sodium and chloride. Expand
The Epstein-Barr virus (EBV) small RNA EBER1 binds and relocalizes ribosomal protein L22 in EBV-infected human B lymphocytes.
TLDR
It is reported that a 15-kDa cellular protein called EAP (for EBER associated protein), previously shown to bind EBER1, is in fact the ribosomal protein L22, and in situ hybridization indicates that the EBER RNPs are predominantly nucleoplasmic, suggesting that L22 relocalization correlates with binding to Eber1 in vivo. Expand
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