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Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunity
TLDR
The replication-defective adenovirus is a promising vaccine vector for development of an HIV-1 vaccine and elicited by a replication-incompetent Ad5 vector, used either alone or as a booster inoculation after priming with a DNA vector. Expand
Protein-excipient interactions: mechanisms and biophysical characterization applied to protein formulation development.
TLDR
Key mechanisms of protein-excipient interactions such as electrostatic and cation-pi interactions, preferential hydration, dispersive forces, and hydrogen bonding are presented in the context of different physical states of the formulation. Expand
Comparative Immunogenicity in Rhesus Monkeys of DNA Plasmid, Recombinant Vaccinia Virus, and Replication-Defective Adenovirus Vectors Expressing a Human Immunodeficiency Virus Type 1 gag Gene
TLDR
Results are suggestive of an immunization strategy for humans that is centered on use of the adenovirus vector and in which existing adenavirus immunity may be overcome by combined immunization with adjuvanted DNA and adenvirus vector boosting. Expand
Vaccine instability in the cold chain: mechanisms, analysis and formulation strategies.
TLDR
Key concepts in understanding the causes and mechanisms of vaccine instability are covered including the complex and delicate nature of antigen structures, as well as formulation approaches for stabilization within the vaccine cold chain. Expand
Evaluation of degradation pathways for plasmid DNA in pharmaceutical formulations via accelerated stability studies.
TLDR
The results indicate that free radical oxidation may be a major degradative process for plasmid DNA in pharmaceutical formulations unless specific measures are taken to control it by the addition of free radical scavengers, specific metal ion chelators, or both. Expand
Disassembly and reassembly of yeast-derived recombinant human papillomavirus virus-like particles (HPV VLPs).
TLDR
This development illustrates how the natural, in vivo mechanisms facilitating cell entry and virus replication can be utilized to achieve an optimal, in vitro assembly state of yeast-expressed HPV VLPs. Expand
Stabilization of human papillomavirus virus-like particles by non-ionic surfactants.
TLDR
The introduction of non-ionic surfactants into HPV VLP aqueous solutions provides significantly enhanced stabilization of HPV VLPs against aggregation upon exposure to low salt and protein concentration, as well as protection against surface adsorption and aggregation due to heat stress and physical agitation. Expand
Pharmaceutical and immunological evaluation of a single-shot hepatitis B vaccine formulated with PLGA microspheres.
TLDR
It is concluded that HBsAg can be successfully encapsulated and recovered from the PLGA microspheres and a mixture of aluminum-adjuvanted and PLGA-formulated HBs Ag can auto-boost an immune response in manner comparable to multiple injections of an aluminum-formulation vaccine. Expand
Nature of the interaction of growth factors with suramin.
TLDR
It is concluded that suramin binds to many growth factors and that this induces microaggregation and, in some cases, conformational changes in the growth factor. Expand
Protein instability and immunogenicity: roadblocks to clinical application of injectable protein delivery systems for sustained release.
TLDR
The need for more extensive analytical characterization in relation to concerns about protein instability in injectable drug delivery systems for sustained release is discussed and the potential roles for academic research in further improving protein stability and developing new analytical technologies to detect protein degradation byproducts in novel drug delivery system are described. Expand
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