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Dimethylsphingosine and FTY720 inhibit the SK1 form but activate the SK2 form of sphingosine kinase from rat heart
Fractionation of cytosolic sphingosine kinase (SKase) activity by gel filtration chromatography gave rise to a 96‐kDa peak that contained only the SK2 form of SKase (by Western analysis) and a broad
Sphingosine 1-phosphate is an important endogenous cardioprotectant released by ischemic pre- and postconditioning.
TLDR
S1P is an important mediator of both IPC and IPOST that is released along with adenosine during each cycle of IPC or IPOST and protects by binding to S1P GPCRs.
Characterization of the acyl-CoA:amino acid N-acyltransferases from primate liver mitochondria.
TLDR
It was concluded that the human and bovine ArAlk are not significantly different, and an AAc purified from rhesus monkey liver found to have similar kinetic constants to the human form indicates that nonprimate enzymes do not have a defect in glutamine conjugation.
Characterization of the CoA ligases of human liver mitochondria catalyzing the activation of short- and medium-chain fatty acids and xenobiotic carboxylic acids.
TLDR
Two distinct forms of xenobiotic/medium-chain fatty acid:CoA ligase (XM-ligase) were isolated from human liver mitochondria and compared with the previously characterized bovine XM-ligases indicated that they were kinetically distinct.
Regulation of microsomal enzymes by phospholipids. V. Kinetic studies of hepatic uridine diphosphate-glucuronyltransferase.
  • D. Vessey, D. Zakim
  • Chemistry, Medicine
    The Journal of biological chemistry
  • 25 May 1972
TLDR
It was observed that high concentrations of p-nitrophenol and o-aminophenol have nonspecific activating effects on UDP-glucuronyltransferase, and the importance of these findings for the design and interpretation of kinetic experiments is discussed.
Differential activation and inhibition of different forms of rat liver glutathione S-transferase by the herbicides 2,4-dichlorophenoxyacetate (2,4-D) and 2,4,5-trichlorophenoxyacetate (2,4,5-T).
TLDR
The 30% inhibition of YaYa caused by 2,4,5-T was shown to reduce the nearly complete inhibition caused by a previously characterized inhibitor, namely bile acids, suggesting competition for a common binding site on the enzyme.
A sphingosine kinase 1 mutation sensitizes the myocardium to ischemia/reperfusion injury.
TLDR
Deletion of the SphK1 gene sensitizes the myocardium to IR injury and appears to impair the protective effect of IPC, providing the first genetic evidence that the Sphk1-S1P pathway is a critical mediator of I PC and cell survival.
Isolation and characterization of mitochondrial acyl-CoA: glycine N-acyltransferases from kidney.
TLDR
When bovine kidney mitochondria were assayed in the presence of Triton X-100, they were found to contain glycine N-acyltransferase activity toward the CoA-adducts of benzoate, butyrate, isovalerate, naphthylacetate, phenylacetates, and salicylate, a major difference in the relative expression of the aralkyl and arylacetyl transferases between liver and kidney.
Role of sphingosine kinase activity in protection of heart against ischemia reperfusion injury.
TLDR
A role for SKase activity in recovery of hemodynamic function after ischemic injury and also in the cardioprotective effect of preconditioning is supported.
Deletion of the sphingosine kinase-1 gene influences cell fate during hypoxia and glucose deprivation in adult mouse cardiomyocytes.
TLDR
It is concluded that S1P generated by GM-1 treatment must be exported from the cell and acts in a paracrine or autocrine manner to couple with its cognate receptor.
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