Structural features of low-molecular-weight heparins affecting their affinity to antithrombin.
- A. Bisio, D. Vecchietti, G. Torri
- BiologyThrombosis and Haemostasis
- 21 September 2009
Enoxaparin is consistently richer in shorter oligosaccharides than Tinzaparin and Dalteparin, and this study is critical for establishing correlations between structural features of LMWHs and their AT-mediated anticoagulant activity.
Determination of the Molecular Weight of Low-Molecular-Weight Heparins by Using High-Pressure Size Exclusion Chromatography on Line with a Triple Detector Array and Conventional Methods
- A. Bisio, A. Mantegazza, S. Bertini
- ChemistryMolecules
- 1 March 2015
The detection of the lower molecular weight components turned out to be the most critical aspect and may underestimate species under 2 KDa when present in low concentration, other methods appeared to emphasize their content.
A shotgun antisense approach to the identification of novel essential genes in Pseudomonas aeruginosa
- R. Rusmini, D. Vecchietti, Raffaella Macchi, Faustino Vidal-Aroca, G. Bertoni
- BiologyBMC Microbiology
- 5 February 2014
Interestingly, the essential genes in this panel were suggested to take part in a broader range of cellular functions than those currently targeted by extant antibiotics, namely protein secretion, biosynthesis of cofactors, prosthetic groups and carriers, energy metabolism, central intermediary metabolism, transport of small molecules, translation, post-translational modification, non-ribosomal peptide synthesis, lipopolysaccharide synthesis/modification, and transcription regulation.
The naked cell: emerging properties of a surfome-streamlined Pseudomonas putida strain
- E. Martínez-García, Sofía Fraile, D. R. Espeso, D. Vecchietti, G. Bertoni, V. de Lorenzo
- BiologybioRxiv
- 17 May 2020
The editing of the genome of Pseudomonas putida was reported for stripping the cells of most outer-facing structures of the bacterial envelope that mediate motion, binding to surfaces and biofilm formation, resulting in the surface-naked strain EM371, the physicochemical properties of which changed dramatically in respect to the wild type counterpart.
TgpA, a Protein with a Eukaryotic-Like Transglutaminase Domain, Plays a Critical Role in the Viability of Pseudomonas aeruginosa
- Andrea Milani, D. Vecchietti, R. Rusmini, G. Bertoni
- Biology, EngineeringPLoS ONE
- 27 November 2012
It is shown that the periplasmic portion of the PA2873 protein, which is named TgpA, does possess transglutaminase activity in vitro, the first report of transglUTaminase Activity in P. aeruginosa.
Analysis of Pseudomonas aeruginosa Cell Envelope Proteome by Capture of Surface-Exposed Proteins on Activated Magnetic Nanoparticles
- D. Vecchietti, D. Di Silvestre, G. Bertoni
- BiologyPLoS ONE
- 30 November 2012
The magneto-capture procedure is simple, safe, and rapid, and appears to be well-suited for envelope studies in highly pathogenic bacteria.
The naked bacterium: emerging properties of a surfome-streamlined Pseudomonas putida strain.
- E. Martínez-García, Sofía Fraile, David Rodríguez Espeso, D. Vecchietti, G. Bertoni, V. de Lorenzo
- BiologyACS Synthetic Biology
- 30 July 2020
The editing of the genome of Pseudomonas putida KT2440 was reported for stripping the cells of most outer-facing structures of the bacterial envelope that mediate motion, binding to surfaces and biofilm formation, resulting in the surface-naked strain EM371, the physical properties of which changed dramatically in respect to the wild type counterpart.
The Landscape of Pseudomonas aeruginosa Membrane-Associated Proteins
- S. Motta, D. Vecchietti, D. Di Silvestre
- BiologyCells
- 1 November 2020
The present study represents one of the widest characterizations of the P. aeruginosa membrane-associated proteome and may facilitates the investigation of other bacterial species whose envelope exposed protein domains are still unknown.
Crystal structure of YeaZ from Pseudomonas aeruginosa.
- D. Vecchietti, S. Ferrara, R. Rusmini, Raffaella Macchi, M. Milani, G. Bertoni
- Biology, ChemistryBiochemical and Biophysical Research…
- 5 February 2016
A shotgun antisense approach to the identification of novel essential genes in Pseudomonas aeruginosa
- R. Rusmini, D. Vecchietti, Raffaella Macchi, Faustino Vidal-Aroca, G. Bertoni
- BiologyBMC Microbiology
- 5 February 2014
Interestingly, the essential genes in this panel were suggested to take part in a broader range of cellular functions than those currently targeted by extant antibiotics, namely protein secretion, biosynthesis of cofactors, prosthetic groups and carriers, energy metabolism, central intermediary metabolism, transport of small molecules, translation, post-translational modification, non-ribosomal peptide synthesis, lipopolysaccharide synthesis/modification, and transcription regulation.
...
...