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Flavin dependent monooxygenases.
TLDR
An update of the classification of flavin-dependent monooxygenases is presented and the latest advances in the understanding of their catalytic and structural properties are summarized. Expand
Identification of a Novel Self-Sufficient Styrene Monooxygenase from Rhodococcus opacus 1CP
TLDR
Cloning and functional expression of His(10)-StyA2B revealed for the first time that the fusion protein does in fact catalyze two separate reactions, strictly NADH-dependent reduction of flavins and highly enantioselective oxygenation of styrene to (S)-styrene oxide were shown. Expand
Gene redundancy of two-component (chloro)phenol hydroxylases in Rhodococcus opacus 1CP.
TLDR
Protein chromatography of phenol- and 4-chlorophenol-grown 1CP-biomass gave first evidences on a functional expression of these oxygenases, which could be initially characterised in respect of their substrate specificity. Expand
Trehalose phosphate synthases OtsA1 and OtsA2 of Rhodococcus opacus 1CP.
TLDR
For the first time, a physiological role of two different OtsAs obtained of a single Rhodococcus strain was presumed and results indicate that OstA2 mainly contributes to the trehalose pool of strain 1CP. Expand
Functional characterization and stability improvement of a ‘thermophilic-like’ ene-reductase from Rhodococcus opacus 1CP
Ene-reductases (ERs) are widely applied for the asymmetric synthesis of relevant industrial chemicals. A novel ER OYERo2 was found within a set of 14 putative old yellow enzymes (OYEs) obtained byExpand
Microbial Styrene Degradation: From Basics to Biotechnology
Styrene, the simplest representative of alkenylbenzenes, is one of the most important monomers produced by the chemical industry today. The compound shows a distinct toxicological behavior and isExpand
Old Yellow Enzyme-Catalysed Asymmetric Hydrogenation: Linking Family Roots with Improved Catalysis
Asymmetric hydrogenation of activated alkenes catalysed by ene-reductases from the old yellow enzyme family (OYEs) leading to chiral products is of potential interest for industrial processes. OYEs’Expand
StyA1 and StyA2B from Rhodococcus opacus 1CP: a Multifunctional Styrene Monooxygenase System
TLDR
It is concluded that the StyA1/StyA2B system represents a novel type of multifunctional flavoprotein monooxygenase that provides new opportunities for biotechnological applications and is highly relevant from a structural and evolutionary point of view. Expand
Biochemical characterization of an azoreductase from Rhodococcus opacus 1CP possessing methyl red degradation ability
Abstract Azo dyes, characterized by one or more R1 N N R2 bonds, could be enzymatically metabolized. A flavin-containing oxygen-insensitive azoreductase designated as AzoRo (25 KDa) from strainExpand
Detection of arsenic-binding siderophores in arsenic-tolerating Actinobacteria by a modified CAS assay.
TLDR
The results suggest that the isolated arsenic-tolerant Actinobacteria produce siderophores that bind arsenic, and open new perspectives on potential candidates for decontaminating environments with arsenic and for other biotechnological applications. Expand
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