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Biochemical Characterization of the Human Copper Transporter Ctr1*
This is the first report on the biochemical characterization of the human copper transporter hCtr1, which is important for understanding mechanisms for mammalian copper transport at the plasma membrane. Expand
Genome-Wide Analysis of the Biology of Stress Responses through Heat Shock Transcription Factor
The whole-genome analyses used to identify virtually all of the direct transcriptional targets of yeast HSF provide novel insights into the role of HSF in growth, development, disease, and aging and in the complex metabolic reprogramming that occurs in all cells in response to stress. Expand
Mechanisms for copper acquisition, distribution and regulation.
Recent advances in the identification and function of cellular and systemic molecules that drive Cu accumulation, distribution and sensing are reviewed. Expand
Uptake of the anticancer drug cisplatin mediated by the copper transporter Ctr1 in yeast and mammals
It is demonstrated that deletion of the yeast CTR1 gene, which encodes a high-affinity copper transporter, results in increased cis platin resistance and reduced intracellular accumulation of cisplatin, and proposed that cisplin uptake is mediated by the copper transporter Ctr1p in yeast and mammals. Expand
Identification of a copper transporter family in Arabidopsis thaliana
A five-member family (COPT1–5) of putative Arabidopsis copper transporters is described, ascertain the ability of these proteins to functionally complement and transport copper in the corresponding Saccharomyces cerevisiae high-affinity copper transport mutant. Expand
Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase.
Overproduction of either SWI5 or ACE2 permits transcriptional activation of the target gene from the other pathway, suggesting that the DNA-binding proteins are capable of binding in vivo to promoters that they do not usually activate. Expand
Redox regulation of mammalian heat shock factor 1 is essential for Hsp gene activation and protection from stress.
It is demonstrated that recombinant mammalian HSF1 directly senses both heat and hydrogen peroxide to assemble into a homotrimer in a reversible and redox-regulated manner. Expand
Biochemical and Genetic Analyses of Yeast and Human High Affinity Copper Transporters Suggest a Conserved Mechanism for Copper Uptake*
The topological orientation of the yeast Ctr1 copper transport protein is elucidated, showing that a series of clustered methionine residues in the hydrophilic extracellular domain and an MXXXM motif in the second transmembrane domain are important for copper uptake but not for protein sorting and delivery to the cell surface. Expand
Copper-specific Transcriptional Repression of Yeast Genes Encoding Critical Components in the Copper Transport Pathway*
  • S. Labbé, Zhiwu Zhu, D. Thiele
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 20 June 1997
Yeast cells coordinately express genes involved in high affinity copper transport through the action of a common signaling pathway, and the Mac1p is essential for down- or up-regulation of the copper-transport genes. Expand
Molecular mechanisms of copper uptake and distribution.
  • S. Puig, D. Thiele
  • Chemistry, Medicine
  • Current opinion in chemical biology
  • 1 April 2002
The recent generation of mouse-knockout models, deficient in a high-affinity copper transporter, or in copper chaperones, has demonstrated the importance of copper uptake and targeted distribution in both predicted and fascinating unanticipated ways in growth and development. Expand