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CUT1, an Arabidopsis Gene Required for Cuticular Wax Biosynthesis and Pollen Fertility, Encodes a Very-Long-Chain Fatty Acid Condensing Enzyme
TLDR
Using the Arabidopsis expressed sequence tag database, a gene is identified that encodes a VLCFA condensing enzyme required for cuticular wax production, and this result indicates that CUT1 is involved in the production of V LCFA precursors used for the synthesis of all stem wax components inArabidopsis. Expand
Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight.
TLDR
The current study confirms the important role of DGAT in regulating the quantity of seed triacylglycerols and the sink size in developing seeds and shows for the first time that seed-specific over-expression of the DGAT cDNA in wild-type Arabidopsis enhances oil deposition and average seed weight, which are correlated with DGAT transcript levels. Expand
The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene.
TLDR
The TAG1 cDNA was over-expressed in yeast, and its activity as a microsomal DGAT confirmed, and the insertion mutation in the TAG1 gene in mutant AS11 results in its altered lipid phenotype. Expand
Alteration of Seed Fatty Acid Composition by an Ethyl Methanesulfonate-Induced Mutation in Arabidopsis thaliana Affecting Diacylglycerol Acyltransferase Activity
TLDR
It is demonstrated that changes to triacylglycerol biosynthesis can result in dramatic changes in fatty acid composition and, in particular, in the accumulation of VLCFAs in seed storage lipids. Expand
Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene.
TLDR
The yeast sn-2 acyltransferase has been shown to encode a protein that can exhibit lysophosphatidic acid acyl transferase activity and that can be used to change total fatty acid content and composition as well as to alter the stereospecific acyl distribution of fatty acids in seed TAGs. Expand
Surface Modification of Poly(lactide-co-glycolide) Nanospheres by Biodegradable Poly(lactide)-Poly(ethylene glycol) Copolymers
TLDR
The in vivo results reveal that coating of PLGA nanospheres with PLA:PEG copolymers can alter the biodistribution in comparison to uncoated PLGA Nanospheres and may suggest a different stability of the adsorbed layers on these two systems. Expand
Production of fatty acid components of meadowfoam oil in somatic soybean embryos.
TLDR
To partially reconstruct the biosynthetic pathway of 20:1Delta(5) in transgenic plant tissues, cDNAs for the L. douglasii acyl-CoA desaturase and FAE1 were co-expressed in somatic soybean embryos. Expand
A simple enzymatic method for the preparation of radiolabeled erucoyl-CoA and other long-chain fatty acyl-CoAs and their characterization by mass spectrometry.
TLDR
Fast ion bombardment-mass spectrometry was employed to confirm the structures of the various acyl-CoAs and yields were greater than 90% and the purities greater than 95%, based on the distribution of radioactivity, and chromatographic and spectral properties. Expand
Induction of Lipid and Oleosin Biosynthesis by (+)-Abscisic Acid and Its Metabolites in Microspore-Derived Embryos of Brassica napus L.cv Reston (Biological Responses in the Presence of
TLDR
To the authors' knowledge, this is the first report of the biological activity of 8[prime]-OH ABA and of stimulatory effects of (+)-ABA and 8[ prime]- OH ABA on lipid and oleosin biosynthesis. Expand
The plastidic phosphoglucomutase from Arabidopsis. A reversible enzyme reaction with an important role in metabolic control.
TLDR
The results indicate that plastidic PGM is an important factor affecting carbon flux in triacylglycerol accumulation in oilseed plants, most likely through its essential role in starch synthesis. Expand
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