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Mechanism for acute control of fatty acid synthesis by glucagon and 3':5'-cyclic AMP in the liver cell.
Labeling experiments with chicken liver cell monolayers and suspensions show that glucagon and N6, O2-dibutyryladenosine 3':5'-cyclic monophosphate (dibutyryl cyclic AMP) block fatty acid synthesisExpand
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Lipogenesis and the synthesis and secretion of very low density lipoprotein by avian liver cells in nonproliferating monolayer culture. Hormonal effects
The nonproliferating chicken liver cell culture system described yields cell monolayers with morphological and lipogenic properties characteristic of the physiological-nutritional state of donorExpand
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Automated fluorometric procedure for measurement of creatine phosphokinase activity.
An automated procedure is described for measuring creatine phosphokinase activity (in serum, although the technique is adaptable) with the Technicon AutoAnalyzer. Creatine, formed by the action ofExpand
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Multiple phosphorylation of acetyl-CoA carboxylase in chick liver cells. A cyclic AMP-independent process.
When chick liver cells in monolayer culture were incubated with 32Pi in the presence of insulin, acetyl-CoA carboxylase became extensively labeled with 32Pi reaching a stoichiometry of 9 to 10 mol ofExpand
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Optimized Determination of γ-Glutamyltransferase by Reaction-Rate Analysis
We describe a method for measuring γ-glutamyltransferase (EC 2.3.2.2) activity in serum, which can be used with automated enzyme analyzers (such as the LKB 8600 Reaction Rate Analyzer) that requireExpand
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Electrophoresis of plasma lipoproteins.
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Kits for Enzyme Activity Determination
There exists a considerable array of commercially prepared kits for enzyme activity determination. This wide choice retlects both the increasing number of enzyme determinations carried out in theExpand
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Amylase Determination Using Insoluble Substrates
Three insoluble substrate amylase methods are compared. There is good correlation between the three procedures and with a saccharogenic procedure. Correlation with the starch-iodine method of CarawayExpand
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