• Publications
  • Influence
Active site remodeling accompanies thioester bond formation in the SUMO E1
E1 enzymes activate ubiquitin (Ub) and ubiquitin-like (Ubl) proteins in two steps by carboxy-terminal adenylation and thioester bond formation to a conserved catalytic cysteine in the E1 Cys domain.Expand
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Small-molecule inhibition of siderophore biosynthesis in Mycobacterium tuberculosis and Yersinia pestis
Mycobacterium tuberculosis and Yersinia pestis, the causative agents of tuberculosis and plague, respectively, are pathogens with serious ongoing impact on global public health1,2 and potential useExpand
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Stereoselective Synthesis of over Two Million Compounds Having Structural Features Both Reminiscent of Natural Products and Compatible with Miniaturized Cell-Based Assays
Understanding the cellular function of a protein usually requires a means to alter the function. This is commonly done by mutating the gene encoding the protein (genetic approach). It can also beExpand
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Expanding the range of 'druggable' targets with natural product-based libraries: an academic perspective.
Existing drugs address a relatively narrow range of biological targets. As a result, libraries of drug-like molecules have proven ineffective against a variety of challenging targets, such asExpand
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Mycobacterial phenolic glycolipid virulence factor biosynthesis: mechanism and small-molecule inhibition of polyketide chain initiation.
Phenolic glycolipids (PGLs) are polyketide-derived virulence factors produced by Mycobacterium tuberculosis, M. leprae, and other mycobacterial pathogens. We have combined bioinformatic, genetic,Expand
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Diversity-oriented synthesis: exploring the intersections between chemistry and biology
  • D. Tan
  • Biology, Chemistry
  • Nature chemical biology
  • 1 July 2005
Diversity-oriented synthesis (DOS) is an emerging field involving the synthesis of combinatorial libraries of diverse small molecules for biological screening. Rather than being directed toward aExpand
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Diversity-Oriented Synthesis Approach to Macrocycles via Oxidative Ring Expansion
Macrocycles are key structural elements in numerous bioactive small molecules and are attractive targets in the diversity-oriented synthesis of natural product-based libraries. However, efficient andExpand
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Mechanism-based inhibitors of MenE, an acyl-CoA synthetase involved in bacterial menaquinone biosynthesis.
Menaquinone (vitamin K(2)) is an essential component of the electron transfer chain in many pathogens, including Mycobacterium tuberculosis and Staphylococcus aureus, and menaquinone biosynthesis isExpand
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A new approach of forecasting intermittent demand for spare parts inventories in the process industries
TLDR
We show that our method produces more accurate forecasts of lead time demands than exponential smoothing, Croston's method and Markov bootstrapping method. Expand
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Designed Small-Molecule Inhibitors of the Anthranilyl-CoA Synthetase PqsA Block Quinolone Biosynthesis in Pseudomonas aeruginosa
The Gram-negative bacterial pathogen Pseudomonas aeruginosa uses three interconnected intercellular signaling systems regulated by the transcription factors LasR, RhlR, and MvfR (PqsR), which mediateExpand
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