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Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis
Using mouse models with tagged mammary tumors, it is demonstrated that CTC clusters arise from oligoclonal tumor cell groupings and not from intravascular aggregation events, and though rare in the circulation, they greatly contribute to the metastatic spread of cancer. Expand
Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
A high-throughput microfluidic mixing device, the herringbone-chip, or “HB-Chip,” is described, which provides an enhanced platform for CTC isolation and reveals microclusters of CTCs, previously unappreciated tumor cell aggregates that may contribute to the hematogenous dissemination of cancer. Expand
Inertial Focusing for Tumor Antigen–Dependent and –Independent Sorting of Rare Circulating Tumor Cells
A multistage microfluidic device that is capable of sorting rare circulating tumor cells (CTCs) that are either positive or negative for the surface antigen epithelial cell adhesion molecule (EpCAM) and could be a promising addition to current diagnostic tools used in the clinic is developed. Expand
RNA-Seq of single prostate CTCs implicates noncanonical Wnt signaling in antiandrogen resistance
Analysis of circulating tumor cells from prostate cancer patients reveals a mechanism that contributes to treatment failure, and a non-invasive method to spot resistance by sequencing RNA transcripts in single circulating tumor Cells (CTCs) is developed. Expand
Isolation and Characterization of Circulating Tumor Cells from Patients with Localized and Metastatic Prostate Cancer
A silicon microfluidic cell-capture technology that, when coupled to an automated imaging system, enables the detection and enumeration of prostate cancer cells fished out from the blood, taking advantage of prostate-specific antigen (PSA), a unique prostate tumor–associated marker. Expand
A microfluidic device for label-free, physical capture of circulating tumor cell-clusters
A microchip technology (the Cluster-Chip) is developed to capture CTC clusters independently of tumor-specific markers from unprocessed blood to enable the detailed characterization of their biological properties and role in metastasis. Expand
Comparison of three directly coupled HPLC MS/MS strategies for identification of proteins from complex mixtures: single-dimension LC-MS/MS, 2-phase MudPIT, and 3-phase MudPIT
Abstract One of the most effective methods for the direct identification of proteins from complex mixtures without first having to resolve them by polyacrylamide gel electrophoresis is to separateExpand
Single-Cell RNA Sequencing Identifies Extracellular Matrix Gene Expression by Pancreatic Circulating Tumor Cells
Mouse as well as human pancreatic CTCs exhibit a very high expression of stromal-derived extracellular matrix (ECM) proteins, including SPARC, whose knockdown in cancer cells suppresses cell migration and invasiveness, pointing to their contribution of microenvironmental signals for the spread of cancer to distant organs. Expand
The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles
The results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles, and pharmacological inhibition of Eg5 results in a dose–responsive slowing of flux, and biochemical depletion of Eg 5 significantly decreases the flux rate. Expand
Androgen receptor signaling in circulating tumor cells as a marker of hormonally responsive prostate cancer.
Microfluidic capture of circulating tumor cells (CTC) is used to measure AR signaling readouts before and after therapeutic interventions to help target treatments to patients most likely to respond to second-line therapies. Expand