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Post Hatching Development: a Novel System for Extended in Vitro Culture of Bovine Embryos
TLDR
A stable culture system of post hatching embryos was first defined and can be used as a model for rapid growth, elongation, and initial differentiation of bovine post hatch embryos produced entirely in vitro.
Detection of bovine viral diarrhea virus in semen obtained after inoculation of seronegative postpubertal bulls.
TLDR
Bovine viral diarrhea virus can persist in semen of acutely infected bulls for several months after exposure, and is detected in serum of all bulls.
The effect of infectious bovine rhinotracheitis vaccine on reproductive efficiency in cattle vaccinated during estrus.
TLDR
The results suggest that the intramuscular inoculation of modified live IBR virus vaccines into cattle during estrus is contraindicated.
Biosecurity issues associated with current and emerging embryo technologies.
TLDR
Details of the risks associated with introducing bovine viral diarrhoea virus in each of three generations of embryo technologies are described and the issue of environmental changes as they relate to the emergence of biohazards and the implementation of biosecurity measures are described.
Necrotic oophoritis in heifers vaccinated intravenously with infectious bovine rhinotracheitis virus vaccine during estrus.
TLDR
Hereford heifers injected IM with prostaglandin F2 alpha a, 11 days apart to synchronize estrous cycles had varying amounts of necrotic and inflammatory change, but the changes appeared to be more severe in 1 group than in the other 2.
Bovine viral diarrhea virus (BVDV): epidemiologic concerns relative to semen and embryos.
TLDR
An overview of the risks of transmitting this virus by AI or production and transfer of embryos and summarizes the precautions needed to prevent such transmissions of disease from occurring is provided.
Comparison of in vitro and in vivo biological activity of mycotoxins.
TLDR
In vitro assays developed to screen the cytotoxic activity of chemicals in murine and bovine embryonic cells found Verrucarin A and roridin A had the strongest cytotoxicity activity, and ergotamine tartrate was least toxic.
Epidemiology of prolonged testicular infections with bovine viral diarrhea virus.
TLDR
It is demonstrated that prolonged testicular infections could result in detection of viral RNA in semen for 2.75 years with infectious virus grown from testicular tissue 12.5 months after viral exposure.
Trypsin treatment of bovine embryos after in vitro exposure to infectious bovine rhinotracheitis virus or bovine herpesvirus-4.
TLDR
The study confirmed previous reports that IBRV adheres to the bovine ZP after in vitro exposure and that trypsin treatment is effective in keeping ZP-I embryos free of this virus.
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